2J04

The tau60-tau91 subcomplex of yeast transcription factor IIIC

2J04 の概要

• エントリーDOI: 10.2210/pdb2j04/pdb
• 分子名称: HYPOTHETICAL PROTEIN YPL007C, YDR362CP (3 entities in total)
• 機能のキーワード: beta propeller, type 2 promoters, transcription, hypothetical protein, preinitiation complex, yeast rna polymerase iii, transcription factor iiic
• 由来する生物種: SACCHAROMYCES CEREVISIAE (BAKER'S YEAST); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 253368.09

構造登録者

Mylona, A.,Fernandez-Tornero, C.,Legrand, P.,Muller, C.W. (登録日: 2006-07-31, 公開日: 2006-10-23, 最終更新日: 2024-11-06)

主引用文献

Mylona, A.,Fernandez-Tornero, C.,Legrand, P.,Haupt, M.,Sentenac, A.,Acker, J.,Muller, C.W.
Structure of the Tau60/Deltatau91 Subcomplex of Yeast Transcription Factor Iiic: Insights Into Preinitiation Complex Assembly
Mol.Cell, 24:221-, 2006

PubMed Abstract: Yeast RNA polymerase III is recruited upon binding of subcomplexes tauA and tauB of transcription factor IIIC (TFIIIC) to the A and B blocks of tRNA gene promoters. The tauB subcomplex consists of subunits tau60, tau91, and tau138. We determined the 3.2 A crystal structure of tau60 bound to a large C-terminal fragment of tau91 (Deltatau91). Deltatau91 protein contains a seven-bladed propeller preceded by an N-terminal extension, whereas tau60 contains a structurally homologous propeller followed by a C-terminal domain with a novel alpha/beta fold. The two propeller domains do not have any detectable DNA binding activity and mediate heterodimer formation that may serve as scaffold for tau138 assembly. We show that the C-terminal tau60 domain interacts with the TATA binding protein (TBP). Recombinant tauB recruits TBP and stimulates TFIIIB-directed transcription on a TATA box containing tRNA gene, implying a combined contribution of tauA and tauB to preinitiation complex formation.

PubMed: 17052456
DOI: 10.1016/J.MOLCEL.2006.08.013

実験手法

X-RAY DIFFRACTION (3.2 Å)