2IZ3

Solution structure of human beta-microseminoprotein

2IZ3 の概要

• エントリーDOI: 10.2210/pdb2iz3/pdb
• 関連するPDBエントリー: 2IZ4
• NMR情報: BMRB: 15037
• 分子名称: BETA-MICROSEMINOPROTEIN (1 entity in total)
• 機能のキーワード: polymorphism, alternative splicing, inhibitor
• 由来する生物種: HOMO SAPIENS (HUMAN)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10955.43

構造登録者

Ghasriani, H.,Teilum, K.,Johnsson, Y.,Fernlund, P.,Drakenberg, T. (登録日: 2006-07-24, 公開日: 2006-10-24, 最終更新日: 2024-10-23)

主引用文献

Ghasriani, H.,Teilum, K.,Johnsson, Y.,Fernlund, P.,Drakenberg, T.
Solution Structure of Human and Porcine Beta-Microseminoprotein
J.Mol.Biol., 362:502-, 2006

PubMed Abstract: Beta-microseminoprotein (MSP) is a small cysteine-rich protein (molecular mass about 10 kDa) first isolated from human seminal plasma and later identified in several other organisms. The function of MSP is not known, but a recent study has shown MSP to bind CRISP-3, a protein present in neutrophilic granulocytes. The amino acid sequence is highly variable between species raising the question of the evolutionary conservation of the 3D structure. Here we present NMR solution structures of both the human and the porcine MSP. The two proteins (sequence identity 51%) have a very similar 3D structure with the secondary structure elements well conserved and with most of the amino acid substitutions causing a change of charge localized to one side of the molecule. MSP is a beta-sheet-rich protein with two distinct domains. The N-terminal domain is composed of a four-stranded beta-sheet, with the strands arranged according to the Greek key-motif, and a less structured part. The C-terminal domain contains two two-stranded beta-sheets with no resemblance to known structural motifs. The two domains, connected to each other by the peptide backbone, one disulfide bond, and interactions between the N and C termini, are oriented to give the molecule a rather extended structure. This global fold differs markedly from that of a previously published structure for porcine MSP, in which the two domains have an entirely different orientation to each other. The difference probably stems from a misinterpretation of ten specific inter-domain NOEs.

PubMed: 16930619
DOI: 10.1016/J.JMB.2006.07.029

実験手法

SOLUTION NMR