2IX8

MODEL FOR EEF3 BOUND TO AN 80S RIBOSOME

Summary for 2IX8

• Entry DOI: 10.2210/pdb2ix8/pdb
• EMDB information: 1233
• Descriptor: ELONGATION FACTOR 3A (1 entity in total)
• Functional Keywords: nucleotide-binding, protein biosynthesis, phosphorylation, elongation factor, rna-binding, atp-binding, rrna-binding
• Biological source: SACCHAROMYCES CEREVISIAE (BAKER'S YEAST)
• Cellular location: Cytoplasm : P16521
• Total number of polymer chains: 1
• Total formula weight: 109441.55

Authors

Andersen, C.B.F.,Becker, T.,Blau, M.,Anand, M.,Halic, M.,Balar, B.,Mielke, T.,Boesen, T.,Pedersen, J.S.,Spahn, C.M.T.,Kinzy, T.G.,Andersen, G.R.,Beckmann, R. (deposition date: 2006-07-07, release date: 2007-07-10, Last modification date: 2024-10-16)

Primary citation

Andersen, C.B.F.,Becker, T.,Blau, M.,Anand, M.,Halic, M.,Balar, B.,Mielke, T.,Boesen, T.,Pedersen, J.S.,Spahn, C.M.T.,Kinzy, T.G.,Andersen, G.R.,Beckmann, R.
Structure of Eef3 and the Mechanism of Transfer RNA Release from the E-Site.
Nature, 443:663-668, 2006

PubMed Abstract: Elongation factor eEF3 is an ATPase that, in addition to the two canonical factors eEF1A and eEF2, serves an essential function in the translation cycle of fungi. eEF3 is required for the binding of the aminoacyl-tRNA-eEF1A-GTP ternary complex to the ribosomal A-site and has been suggested to facilitate the clearance of deacyl-tRNA from the E-site. Here we present the crystal structure of Saccharomyces cerevisiae eEF3, showing that it consists of an amino-terminal HEAT repeat domain, followed by a four-helix bundle and two ABC-type ATPase domains, with a chromodomain inserted in ABC2. Moreover, we present the cryo-electron microscopy structure of the ATP-bound form of eEF3 in complex with the post-translocational-state 80S ribosome from yeast. eEF3 uses an entirely new factor binding site near the ribosomal E-site, with the chromodomain likely to stabilize the ribosomal L1 stalk in an open conformation, thus allowing tRNA release.

PubMed: 16929303
DOI: 10.1038/nature05126

Experimental method

ELECTRON MICROSCOPY (9.9 Å)