2IVF

Ethylbenzene dehydrogenase from Aromatoleum aromaticum

2IVF の概要

• エントリーDOI: 10.2210/pdb2ivf/pdb
• 分子名称: ETHYLBENZENE DEHYDROGENASE ALPHA-SUBUNIT, PHOSPHORIC ACID 4-(2-AMINO-4-OXO-3,4,5,6,-TETRAHYDRO-PTERIDIN-6-YL)-2-HYDROXY-3,4-DIMERCAPTO-BUT-3-EN-YL ESTER GUANYLATE ESTER, PHOSPHATE ION, ... (14 entities in total)
• 機能のキーワード: anaerobic hydrocarbon degradation, moco, fe/s cluster, mo- bismgd enzyme, dmso reductase family, oxidoreductase
• 由来する生物種: AROMATOLEUM AROMATICUM; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 178274.38

構造登録者

Kloer, D.P.,Hagel, C.,Heider, J.,Schulz, G.E. (登録日: 2006-06-13, 公開日: 2006-09-14, 最終更新日: 2024-05-08)

主引用文献

Kloer, D.P.,Hagel, C.,Heider, J.,Schulz, G.E.
Crystal Structure of Ethylbenzene Dehydrogenase from Aromatoleum Aromaticum
Structure, 14:1377-, 2006

PubMed Abstract: Anaerobic degradation of hydrocarbons was discovered a decade ago, and ethylbenzene dehydrogenase was one of the first characterized enzymes involved. The structure of the soluble periplasmic 165 kDa enzyme was established at 1.88 A resolution. It is a heterotrimer. The alpha subunit contains the catalytic center with a molybdenum held by two molybdopterin-guanine dinucleotides, one with an open pyran ring, and an iron-sulfur cluster with a histidine ligand. During catalysis, electrons produced by substrate oxidation are transferred to a heme in the gamma subunit and then presumably to a separate cytochrome involved in nitrate respiration. The beta subunit contains four iron-sulfur clusters and is structurally related to ferredoxins. The gamma subunit is the first known protein with a methionine and a lysine as axial heme ligands. The catalytic product was modeled into the active center, showing the reaction geometry. A mechanism consistent with activity and inhibition data of ethylbenzene-related compounds is proposed.

PubMed: 16962969
DOI: 10.1016/J.STR.2006.07.001

実験手法

X-RAY DIFFRACTION (1.88 Å)