2ITF

Crystal structure IsdA NEAT domain from Staphylococcus aureus with heme bound

2ITF の概要

• エントリーDOI: 10.2210/pdb2itf/pdb
• 関連するPDBエントリー: 2itf
• 分子名称: Iron-regulated surface determinant protein A, PROTOPORPHYRIN IX CONTAINING FE (3 entities in total)
• 機能のキーワード: neat domain, heme, iron, metal binding protein
• 由来する生物種: Staphylococcus aureus
• 細胞内の位置: Secreted, cell wall ; Peptidoglycan-anchor : Q7A152
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 61614.04

構造登録者

Grigg, J.C.,Vermeiren, C.L.,Heinrichs, D.E.,Murphy, M.E. (登録日: 2006-10-19, 公開日: 2006-12-26, 最終更新日: 2024-10-30)

主引用文献

Grigg, J.C.,Vermeiren, C.L.,Heinrichs, D.E.,Murphy, M.E.
Haem recognition by a Staphylococcus aureus NEAT domain.
Mol.Microbiol., 63:139-149, 2007

PubMed Abstract: Successful pathogenic organisms have developed mechanisms to thrive under extreme levels of iron restriction. Haem-iron represents the largest iron reservoir in the human body and is a significant source of iron for some bacterial pathogens. NEAT (NEAr Transporter) domains are found exclusively in a family of cell surface proteins in Gram-positive bacteria. Many NEAT domain-containing proteins, including IsdA in Staphylococcus aureus, are implicated in haem binding. Here, we show that overexpression of IsdA in S. aureus enhances growth and an inactivation mutant of IsdA has a growth defect, compared with wild type, when grown in media containing haem as the sole iron source. Furthermore, the haem-binding property of IsdA is contained within the NEAT domain. Crystal structures of the apo-IsdA NEAT domain and in complex with haem were solved and reveal a clathrin adapter-like beta-sandwich fold with a large hydrophobic haem-binding pocket. Haem is bound with the propionate groups directed at the molecular surface and the iron is co-ordinated solely by Tyr(166). The phenol groups of Tyr(166) and Tyr(170) form an H-bond that may function in regulating haem binding and release. An analysis of IsdA structure-sequence alignments indicate that conservation of Tyr(166) is a predictor of haem binding by NEAT domains.

PubMed: 17229211
DOI: 10.1111/j.1365-2958.2006.05502.x

実験手法

X-RAY DIFFRACTION (1.9 Å)