2INX

Crystal Structure of Ketosteroid Isomerase D40N from Pseudomonas putida (pKSI) with bound 2,6-difluorophenol

2INX の概要

• エントリーDOI: 10.2210/pdb2inx/pdb
• 分子名称: Steroid delta-isomerase, 2,6-DIFLUOROPHENOL (3 entities in total)
• 機能のキーワード: ksi, enzyme, active site, charge distribution, hydrogen bond, isomerase
• 由来する生物種: Pseudomonas putida
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14677.61

構造登録者

Martinez Caaveiro, J.M.,Pybus, B.,Ringe, D.,Petsko, G.A.,Sigala, P.,Kraut, D.,Herschlag, D. (登録日: 2006-10-09, 公開日: 2007-10-23, 最終更新日: 2023-08-30)

主引用文献

Sigala, P.A.,Kraut, D.A.,Caaveiro, J.M.,Pybus, B.,Ruben, E.A.,Ringe, D.,Petsko, G.A.,Herschlag, D.
Testing geometrical discrimination within an enzyme active site: constrained hydrogen bonding in the ketosteroid isomerase oxyanion hole.
J.Am.Chem.Soc., 130:13696-13708, 2008

PubMed Abstract: Enzymes are classically proposed to accelerate reactions by binding substrates within active-site environments that are structurally preorganized to optimize binding interactions with reaction transition states rather than ground states. This is a remarkably formidable task considering the limited 0.1-1 A scale of most substrate rearrangements. The flexibility of active-site functional groups along the coordinate of substrate rearrangement, the distance scale on which enzymes can distinguish structural rearrangement, and the energetic significance of discrimination on that scale remain open questions that are fundamental to a basic physical understanding of enzyme active sites and catalysis. We bring together 1.2-1.5 A resolution X-ray crystallography, (1)H and (19)F NMR spectroscopy, quantum mechanical calculations, and transition-state analogue binding measurements to test the distance scale on which noncovalent forces can constrain the structural relaxation or translation of side chains and ligands along a specific coordinate and the energetic consequences of such geometric constraints within the active site of bacterial ketosteroid isomerase (KSI). Our results strongly suggest that packing and binding interactions within the KSI active site can constrain local side-chain reorientation and prevent hydrogen bond shortening by 0.1 A or less. Further, this constraint has substantial energetic effects on ligand binding and stabilization of negative charge within the oxyanion hole. These results provide evidence that subtle geometric effects, indistinguishable in most X-ray crystallographic structures, can have significant energetic consequences and highlight the importance of using synergistic experimental approaches to dissect enzyme function.

PubMed: 18808119
DOI: 10.1021/ja803928m

実験手法

X-RAY DIFFRACTION (1.5 Å)