2INT

CRYSTAL STRUCTURE OF RECOMBINANT HUMAN INTERLEUKIN-4

Summary for 2INT

• Entry DOI: 10.2210/pdb2int/pdb
• Descriptor: INTERLEUKIN-4 (1 entity in total)
• Functional Keywords: cytokine
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 1
• Total formula weight: 14989.25

Authors

Walter, M.R.,Cook, W.J.,Zhao, B.G.,Cameron Junior, R.,Ealick, S.E.,Walter Junior, R.L.,Reichert, P.,Nagabhushan, T.L.,Trotta, P.P.,Bugg, C.E. (deposition date: 1993-07-22, release date: 1994-01-31, Last modification date: 2024-11-13)

Primary citation

Walter, M.R.,Cook, W.J.,Zhao, B.G.,Cameron Jr., R.P.,Ealick, S.E.,Walter Jr., R.L.,Reichert, P.,Nagabhushan, T.L.,Trotta, P.P.,Bugg, C.E.
Crystal structure of recombinant human interleukin-4.
J.Biol.Chem., 267:20371-20376, 1992

PubMed Abstract: The crystal structure of recombinant human interleukin-4 (rhuIL-4) was initially determined at 3.5-A resolution by multiple isomorphous replacement techniques and subsequently refined to a resolution of 2.35 A by simulated annealing. The final crystallographic R-factor, based on all data in the range 6.0-2.35 A (7470 reflections), is 0.232. Bond lengths and bond angles in the molecule have root mean square deviations from ideal values of 0.016 A and 2.4 degrees, respectively. The overall structure is highly compact and globular with a predominantly hydrophobic core. The main structural feature of rhuIL-4 is a four alpha-helix bundle, which composes approximately 58% of the structure. The helices are arranged in a left-handed antiparallel bundle with two overhand connections. Within these connections is a two-stranded antiparallel beta-sheet. Both the tertiary and secondary structures of rhuIL-4 are similar to those of human granulocyte-macrophage colony-stimulating factor. Critical regions for receptor binding are proposed.

PubMed: 1400355

Experimental method

X-RAY DIFFRACTION (2.35 Å)