2ILN

Crystal structure of the Bowman-Birk inhibitor from snail medic seeds in complex with bovine trypsin

2ILN の概要

• エントリーDOI: 10.2210/pdb2iln/pdb
• 関連するPDBエントリー: 1MVZ
• 分子名称: Cationic trypsin, Bowman-Birk type proteinase inhibitor (3 entities in total)
• 機能のキーワード: medicago scutellata, protease inhibitor, bowman-birk inhibitor, trypsin, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor
• 由来する生物種: Bos taurus (cattle); 詳細
• 細胞内の位置: Secreted, extracellular space: P00760
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 53588.59

構造登録者

Capaldi, S.,Perduca, M.,Faggion, B.,Carrizo, M.E.,Tava, A.,Ragona, L.,Monaco, H.L. (登録日: 2006-10-03, 公開日: 2007-04-10, 最終更新日: 2023-10-25)

主引用文献

Capaldi, S.,Perduca, M.,Faggion, B.,Carrizo, M.E.,Tava, A.,Ragona, L.,Monaco, H.L.
Crystal structure of the anticarcinogenic Bowman-Birk inhibitor from snail medic (Medicago scutellata) seeds complexed with bovine trypsin
J.Struct.Biol., 158:71-79, 2007

PubMed Abstract: The structure of the ternary complex of the anticarcinogenic Bowman-Birk protease inhibitor purified from snail medic (Medicago scutellata) seeds (MSTI) and two molecules of bovine trypsin has been solved by X-ray diffraction analysis of single crystals to a resolution of 2.0 A. This is the highest resolution model of a ternary complex of this type currently available. The two binding loops of the MSTI differ in only one amino acid and have in both cases an arginine in position P1. The distances between the residues of the inhibitor at the binding interface and the trypsin side chains that recognize them are almost identical in the two sites. When compared to the NMR model of the uncomplexed MSTI, the inhibitor in the functional assembly with trypsin shows the largest differences in the two P2' residues. Compared with the similar ternary complex of the soybean trypsin inhibitor, this model shows very small differences in the polypeptide chain of the trypsin binding sites and its largest difference in the area between Asp 26 and His 32 of the MSTI which in the soybean inhibitor has an extra Leu inserted in position 29.

PubMed: 17142058
DOI: 10.1016/j.jsb.2006.10.017

実験手法

X-RAY DIFFRACTION (2 Å)