2IL9

Crystal Structure of Plautia Stali Intestine Virus Intergenic Region Internal Ribosome Entry Site Ribosomal Binding Domain RNA at 3.1 Angstroms

Summary for 2IL9

Entry DOI	10.2210/pdb2il9/pdb
Descriptor	Ribosomal Binding Domain of the IRES RNA (1 entity in total)
Functional Keywords	rna; ires; ribosomal binding domain; psiv; under wound helix; double-nested pseudoknot, rna
Total number of polymer chains	2
Total formula weight	91151.88
Authors	Pfingsten, J.S.,Costantino, D.A.,Kieft, J.S. (deposition date: 2006-10-02, release date: 2007-02-13, Last modification date: 2024-02-21)
Primary citation	Pfingsten, J.S.,Costantino, D.A.,Kieft, J.S. Structural basis for ribosome recruitment and manipulation by a viral IRES RNA Science, 314:1450-1454, 2006 Cited by PubMed Abstract: Canonical cap-dependent translation initiation requires a large number of protein factors that act in a stepwise assembly process. In contrast, internal ribosomal entry sites (IRESs) are cis-acting RNAs that in some cases completely supplant these factors by recruiting and activating the ribosome using a single structured RNA. Here we present the crystal structures of the ribosome-binding domain from a Dicistroviridae intergenic region IRES at 3.1 angstrom resolution, providing a view of the prefolded architecture of an all-RNA translation initiation apparatus. Docking of the structure into cryo-electron microscopy reconstructions of an IRES-ribosome complex suggests a model for ribosome manipulation by a dynamic IRES RNA. PubMed: 17124290 DOI: 10.1126/science.1133281 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (3.1 Å)

Structure validation