2IL9

Crystal Structure of Plautia Stali Intestine Virus Intergenic Region Internal Ribosome Entry Site Ribosomal Binding Domain RNA at 3.1 Angstroms

2IL9 の概要

• エントリーDOI: 10.2210/pdb2il9/pdb
• 分子名称: Ribosomal Binding Domain of the IRES RNA (1 entity in total)
• 機能のキーワード: rna; ires; ribosomal binding domain; psiv; under wound helix; double-nested pseudoknot, rna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 91151.88

構造登録者

Pfingsten, J.S.,Costantino, D.A.,Kieft, J.S. (登録日: 2006-10-02, 公開日: 2007-02-13, 最終更新日: 2024-02-21)

主引用文献

Pfingsten, J.S.,Costantino, D.A.,Kieft, J.S.
Structural basis for ribosome recruitment and manipulation by a viral IRES RNA
Science, 314:1450-1454, 2006

PubMed Abstract: Canonical cap-dependent translation initiation requires a large number of protein factors that act in a stepwise assembly process. In contrast, internal ribosomal entry sites (IRESs) are cis-acting RNAs that in some cases completely supplant these factors by recruiting and activating the ribosome using a single structured RNA. Here we present the crystal structures of the ribosome-binding domain from a Dicistroviridae intergenic region IRES at 3.1 angstrom resolution, providing a view of the prefolded architecture of an all-RNA translation initiation apparatus. Docking of the structure into cryo-electron microscopy reconstructions of an IRES-ribosome complex suggests a model for ribosome manipulation by a dynamic IRES RNA.

PubMed: 17124290
DOI: 10.1126/science.1133281

実験手法

X-RAY DIFFRACTION (3.1 Å)