2II2

Crystal Structure of Alpha-11 Giardin

2II2 の概要

• エントリーDOI: 10.2210/pdb2ii2/pdb
• 関連するPDBエントリー: 2IIC
• 分子名称: Alpha-11 giardin, SULFATE ION (3 entities in total)
• 機能のキーワード: helix-turn-helix, metal binding protein
• 由来する生物種: Giardia intestinalis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 35445.06

構造登録者

Pathuri, P.,Nguyen, E.T.,Luecke, H. (登録日: 2006-09-27, 公開日: 2007-04-17, 最終更新日: 2024-02-21)

主引用文献

Pathuri, P.,Nguyen, E.T.,Svard, S.G.,Luecke, H.
Apo and Calcium-bound Crystal Structures of Alpha-11 Giardin, an Unusual Annexin from Giardia lamblia
J.Mol.Biol., 368:493-508, 2007

PubMed Abstract: Alpha-11 giardin is a member of the multi-gene alpha-giardin family in the intestinal protozoan, Giardia lamblia. This gene family shares an ancestry with the annexin super family, whose common characteristic is calcium-dependent binding to membranes that contain acidic phospholipids. Several alpha giardins are highly expressed during parasite-induced diarrhea in humans. Despite being a member of a large family of proteins, little is known about the function and cellular localization of alpha-11 giardin, although giardins are often associated with the cytoskeleton. It has been shown that Giardia exhibits high levels of alpha-11 giardin mRNA transcript throughout its life cycle; however, constitutive over-expression of this protein is lethal to the parasite. Determining the three-dimensional structure of an alpha-giardin is essential to identifying functional domains shared in the alpha-giardin family. Here we report the crystal structures of the apo and Ca(2+)-bound forms of alpha-11 giardin, the first alpha giardin to be characterized structurally. Crystals of apo and Ca(2+)-bound alpha-11 giardin diffracted to 1.1 A and 2.93 A, respectively. The crystal structure of selenium-substituted apo alpha-11 giardin reveals a planar array of four tandem repeats of predominantly alpha-helical domains, reminiscent of previously determined annexin structures, making this the highest-resolution structure of an annexin to date. The apo alpha-11 giardin structure also reveals a hydrophobic core formed between repeats I/IV and II/III, a region typically hydrophilic in other annexins. Surprisingly, the Ca(2+)-bound structure contains only a single calcium ion, located in the DE loop of repeat I and coordinated differently from the two types of calcium sites observed in previous annexin structures. The apo and Ca(2+)-bound alpha-11 giardin structures assume overall similar conformations; however, Ca(2+)-bound alpha-11 giardin crystallized in a lower-symmetry space group with four molecules in the asymmetric unit. Vesicle-binding studies suggest that alpha-11 giardin, unlike most other annexins, does not bind to vesicles composed of acidic phospholipids in a calcium-dependent manner.

PubMed: 17355882
DOI: 10.1016/j.jmb.2007.02.016

実験手法

X-RAY DIFFRACTION (1.1 Å)