2IF1

HUMAN TRANSLATION INITIATION FACTOR EIF1, NMR, 29 STRUCTURES

2IF1 の概要

• エントリーDOI: 10.2210/pdb2if1/pdb
• NMR情報: BMRB: 4255
• 分子名称: EIF1 (1 entity in total)
• 機能のキーワード: translation initiation factor
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14327.20

構造登録者

Fletcher, C.M.,Hellen, C.U.T.,Pestova, T.V.,Wagner, G. (登録日: 1998-08-04, 公開日: 1999-05-18, 最終更新日: 2024-05-29)

主引用文献

Fletcher, C.M.,Pestova, T.V.,Hellen, C.U.,Wagner, G.
Structure and interactions of the translation initiation factor eIF1.
EMBO J., 18:2631-2637, 1999

PubMed Abstract: eIF1 is a universally conserved translation factor that is necessary for scanning and involved in initiation site selection. We have determined the solution structure of human eIF1 with an N-terminal His tag using NMR spectroscopy. Residues 29-113 of the native sequence form a tightly packed domain with two alpha-helices on one side of a five-stranded parallel and antiparallel beta-sheet. The fold is new but similar to that of several ribosomal proteins and RNA-binding domains. A likely binding site is indicated by yeast mutations and conserved residues located together on the surface. No interaction with recombinant eIF5 or the initiation site RNA GCCACAAUGGCA was detected by NMR, but GST pull-down experiments show that eIF1 binds specifically to the p110 subunit of eIF3. This interaction explains how eIF1 is recruited to the 40S ribosomal subunit.

PubMed: 10228174
DOI: 10.1093/emboj/18.9.2631

実験手法

SOLUTION NMR