2IAK

Crystal Structure of a protease resistant fragment of the plakin domain of Bullous Pemphigoid Antigen1 (BPAG1)

2IAK の概要

• エントリーDOI: 10.2210/pdb2iak/pdb
• 分子名称: Bullous pemphigoid antigen 1, isoform 5, SULFATE ION (3 entities in total)
• 機能のキーワード: triple helical bundle, spectrin repeat, cell adhesion
• 由来する生物種: Mus musculus (house mouse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 26752.07

構造登録者

Jefferson, J.J. (登録日: 2006-09-08, 公開日: 2006-11-28, 最終更新日: 2024-02-21)

主引用文献

Jefferson, J.J.,Ciatto, C.,Shapiro, L.,Liem, R.K.
Structural analysis of the plakin domain of bullous pemphigoid antigen1 (BPAG1) suggests that plakins are members of the spectrin superfamily.
J.Mol.Biol., 366:244-257, 2007

PubMed Abstract: Bullous pemphigoid antigen 1 (BPAG1) is a member of the plakin family of proteins. The plakins are multi-domain proteins that have been shown to interact with microtubules, actin filaments and intermediate filaments, as well as proteins found in cellular junctions. These interactions are mediated through different domains on the plakins. The interactions between plakins and components of specialized cell junctions such as desmosomes and hemidesmosomes are mediated through the so-called plakin domain, which is a common feature of the plakins. We report the crystal structure of a stable fragment from BPAG1, residues 226-448, defined by limited proteolysis of the whole plakin domain. The structure, determined by single-wavelength anomalous diffraction phasing from a selenomethionine-substituted crystal at 3.0 A resolution, reveals a tandem pair of triple helical bundles closely related to spectrin repeats. Based on this structure and analysis of sequence conservation, we propose that the architecture of plakin domains is defined by two pairs of spectrin repeats interrupted by a putative Src-Homology 3 (SH3) domain.

PubMed: 17161423
DOI: 10.1016/j.jmb.2006.11.036

実験手法

X-RAY DIFFRACTION (3 Å)