2IAH

Crystal structure of the ferripyoverdine receptor of the outer membrane of Pseudomonas aeruginosa bound to ferripyoverdine.

2IAH の概要

• エントリーDOI: 10.2210/pdb2iah/pdb
• 関連するPDBエントリー: 1XKH 1XKW
• 関連するBIRD辞書のPRD_ID: PRD_000723
• 分子名称: Ferripyoverdine receptor, Pyoverdin C-E, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: pyoverdine, fpva, tonb box, siderophore, cell membrane, ion transport, tonb dependent receptor, membrane protein
• 由来する生物種: Pseudomonas aeruginosa; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 88081.40

構造登録者

Wirth, C.,Pattus, F.,Cobessi, D. (登録日: 2006-09-08, 公開日: 2007-09-11, 最終更新日: 2023-11-15)

主引用文献

Wirth, C.,Meyer-Klaucke, W.,Pattus, F.,Cobessi, D.
From the periplasmic signaling domain to the extracellular face of an outer membrane signal transducer of Pseudomonas aeruginosa: crystal structure of the ferric pyoverdine outer membrane receptor.
J.Mol.Biol., 368:398-406, 2007

PubMed Abstract: The pyoverdine outer membrane receptor, FpvA, from Pseudomonas aeruginosa translocates ferric pyoverdine across the outer membrane through an energy consuming mechanism using the proton motive force and the TonB-ExbB-ExbD energy transducing complex from the inner membrane. We solved the crystal structure of the full-length FpvA bound to iron-pyoverdine at 2.7 A resolution. Signal transduction to an anti-sigma protein of the inner membrane and to TonB-ExbB-ExbD involves the periplasmic domain, which displays a beta-alpha-beta fold composed of two alpha-helices sandwiched by two beta-sheets. One iron-pyoverdine conformer is bound at the extracellular face of FpvA, revealing the conformer selectivity of the binding site. The loop that contains the TonB box, involved in interactions with TonB, and connects the signaling domain to the plug domain of FpvA is not defined in the electron density following the binding of ferric pyoverdine. The high flexibility of this loop is probably necessary for signal transduction through the outer membrane.

PubMed: 17349657
DOI: 10.1016/j.jmb.2007.02.023

実験手法

X-RAY DIFFRACTION (2.73 Å)