2I7K

Solution Structure of the Bromodomain of Human BRD7 Protein

Summary for 2I7K

• Entry DOI: 10.2210/pdb2i7k/pdb
• NMR Information: BMRB: 7287
• Descriptor: Bromodomain-containing protein 7 (1 entity in total)
• Functional Keywords: helix, left-handed four-helix bundle, transcription
• Biological source: Homo sapiens (human)
• Cellular location: Isoform 2: Nucleus: Q9NPI1
• Total number of polymer chains: 1
• Total formula weight: 13918.07

Authors

Sun, H.,Liu, J.,Zhang, J.,Huang, H.,Wu, J.,Shi, Y. (deposition date: 2006-08-31, release date: 2007-07-10, Last modification date: 2024-05-29)

Primary citation

Sun, H.,Liu, J.,Zhang, J.,Shen, W.,Huang, H.,Xu, C.,Dai, H.,Wu, J.,Shi, Y.
Solution structure of BRD7 bromodomain and its interaction with acetylated peptides from histone H3 and H4
Biochem.Biophys.Res.Commun., 358:435-441, 2007

PubMed Abstract: BRD7 is an important protein tightly associated with Nasopharyngeal carcinoma (NPC). Overexpression of BRD7 inhibits NPC cell growth and cell cycle by transcriptionally regulating the cell cycle related genes. BRD7 contains a bromodomain that is found in many chromatin-associated proteins and in nearly all known nuclear histone acetyltransferases (HATs) and plays an important role in chromatin remodeling and transcriptional activation. Here, we report the solution structure of BRD7 bromodomain determined by NMR spectroscopy, and its binding specificity revealed by NMR titration with several acetylated histone peptides. We find that BRD7 bromodomain contains the typical left-handed four-helix bundle topology, and can bind with weak affinity to lysine-acetylated peptides derived from histone H3 with K9 or K14 acetylated and from histone H4 with K8, K12 or K16 acetylated. Our results show that BRD7 bromodomain lacks inherent binding specificity when binding to histones in vitro.

PubMed: 17498659
DOI: 10.1016/j.bbrc.2007.04.139

Experimental method

SOLUTION NMR