2I7E

GAAA tetralooop receptor complex with associated cobalt hexammine.

2I7E の概要

• エントリーDOI: 10.2210/pdb2i7e/pdb
• 関連するPDBエントリー: 2ADT
• 分子名称: 43-MER, COBALT HEXAMMINE(III) (2 entities in total)
• 機能のキーワード: gaaa tetraloop, 11-nucleotide receptor, rna tertiary structure, ribonucleic acid
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 28390.98

構造登録者

Davis, J.H.,Butcher, S.E. (登録日: 2006-08-30, 公開日: 2007-04-03, 最終更新日: 2024-05-29)

主引用文献

Davis, J.H.,Foster, T.R.,Tonelli, M.,Butcher, S.E.
Role of metal ions in the tetraloop-receptor complex as analyzed by NMR.
Rna, 13:76-86, 2007

PubMed Abstract: Metal ions are critical for the proper folding of RNA, and the GAAA tetraloop-receptor is necessary for the optimal folding and function of many RNAs. We have used NMR to investigate the role of metal ions in the structure of the tetraloop-receptor in solution. The NMR data indicate native tertiary structure is formed under a wide range of ionic conditions. The lack of conformational adaptation in response to very different ionic conditions argues against a structural role for divalent ions. Nuclear Overhauser effects to cobalt hexammine and paramagnetic relaxation enhancement induced by manganese ions were used to determine the NMR structures of the tetraloop receptor in association with metal ions, providing the first atomic-level view of these interactions in the solution state. Five manganese and two cobalt hexammine ions could be localized to the RNA surface. The locations of the associated metal ions are similar, but not identical to, those of previously determined crystal structures. The sites of association are in general agreement with nonlinear Poisson-Boltzmann calculations of the electrostatic surface, emphasizing the general importance of diffusely associated ions in RNA tertiary structure.

PubMed: 17119098
DOI: 10.1261/rna.268307

実験手法

SOLUTION NMR