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2I18

The refined structure of C-terminal domain of an EF-hand Calcium binding Protein from Entamoeba Histolytica

2EV7」から置き換えられました
2I18 の概要
エントリーDOI10.2210/pdb2i18/pdb
関連するPDBエントリー1JFJ 1JFK
分子名称Calcium-binding protein, LANTHANUM (III) ION (2 entities in total)
機能のキーワードbeta sheet, alpha helix, calcium binding loops, metal binding protein
由来する生物種Entamoeba histolytica
タンパク質・核酸の鎖数1
化学式量合計7334.08
構造登録者
Mustafi, S.M.,Mukherjee, S.,Chary, K.V.R. (登録日: 2006-08-13, 公開日: 2006-08-22, 最終更新日: 2024-05-01)
主引用文献Mustafi, S.M.,Mukherjee, S.,Chary, K.V.,Cavallaro, G.
Structural basis for the observed differential magnetic anisotropic tensorial values in calcium binding proteins.
Proteins, 65:656-669, 2006
Cited by
PubMed Abstract: Lanthanide ions (Ln(3+)), which have ionic radii similar to those of Ca(2+), can displace the latter in a calcium binding protein, without affecting its tertiary structure. The paramagnetic Ln(3+) possesses large anisotropic magnetic susceptibilities and produce pseudocontact shifts (PCSs), which have r(-3) dependence. The PCS can be seen for spins as far as 45 A from the paramagnetic ion. They aid in structure refinement of proteins by providing long-range distance constraints. Besides, they can be used to determine the interdomain orientation in multidomain proteins. This is particularly important in the context of a calcium binding protein from Entamoeba histolytica (EhCaBP), which consists of two globular domains connected by a flexible linker region containing 8 residues. As a first step to obtain the interdomain orientation in EhCaBP, a suite of 2D and 3D heteronuclear experiments were recorded on EhCaBP by displacing calcium with Ce(3+), Ho(3+), Er(3+), Tm(3+), Dy(3+), and Yb(3+) ions in separate experiments, and the PCS of (1)H(N) and (15)N spins were measured. Such data have been used in the refinement of the individual domain structures of the protein in parallel with the calculation of the respective magnetic anisotropy tensorial values, which differ substantially (2.1-2.8 times) from what is found in other Ca(2+) binding loops. This study provides a structural basis for such variations in the magnetic anisotropy tensorial values.
PubMed: 16981203
DOI: 10.1002/prot.21121
主引用文献が同じPDBエントリー
実験手法
SOLUTION NMR
構造検証レポート
Validation report summary of 2i18
検証レポート(詳細版)ダウンロードをダウンロード

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件を2024-10-30に公開中

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