2HZD

NMR structure of the DNA-binding TEA domain and insights into TEF-1 function

2HZD の概要

• エントリーDOI: 10.2210/pdb2hzd/pdb
• 分子名称: Transcriptional enhancer factor TEF-1 (1 entity in total)
• 機能のキーワード: dna-binding, helix-turn-helix, gene regulation
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus: P28347
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9549.00

構造登録者

Anbanandam, A.,Veeraraghavan, S. (登録日: 2006-08-08, 公開日: 2006-10-24, 最終更新日: 2024-05-29)

主引用文献

Anbanandam, A.,Albarado, D.C.,Nguyen, C.T.,Halder, G.,Gao, X.,Veeraraghavan, S.
Insights into transcription enhancer factor 1 (TEF-1) activity from the solution structure of the TEA domain.
Proc.Natl.Acad.Sci.Usa, 103:17225-17230, 2006

PubMed Abstract: Transcription enhancer factor 1 is essential for cardiac, skeletal, and smooth muscle development and uses its N-terminal TEA domain (TEAD) to bind M-CAT elements. Here, we present the first structure of TEAD and show that it is a three-helix bundle with a homeodomain fold. Structural data reveal how TEAD binds DNA. Using structure-function correlations, we find that the L1 loop is essential for cooperative loading of TEAD molecules on to tandemly duplicated M-CAT sites. Furthermore, using a microarray chip-based assay, we establish that known binding sites of the full-length protein are only a subset of DNA elements recognized by TEAD. Our results provide a model for understanding the regulation of genome-wide gene expression during development by TEA/ATTS family of transcription factors.

PubMed: 17085591
DOI: 10.1073/pnas.0607171103

実験手法

SOLUTION NMR