2HWE

A COMPARISON OF THE ANTI-RHINOVIRAL DRUG BINDING POCKET IN HRV14 AND HRV1A

2HWE の概要

• エントリーDOI: 10.2210/pdb2hwe/pdb
• 分子名称: HUMAN RHINOVIRUS 1A COAT PROTEIN (SUBUNIT VP1), HUMAN RHINOVIRUS 1A COAT PROTEIN (SUBUNIT VP2), HUMAN RHINOVIRUS 1A COAT PROTEIN (SUBUNIT VP3), ... (5 entities in total)
• 機能のキーワード: rhinovirus coat protein, icosahedral virus, virus
• 由来する生物種: Human rhinovirus 1A; 詳細
• 細胞内の位置: Protein VP2: Virion. Protein VP3: Virion. Protein VP1: Virion: P23008 P23008 P23008 P23008
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 92869.54

構造登録者

Kim, K.H.,Rossmann, M.G. (登録日: 1994-01-25, 公開日: 1994-09-30, 最終更新日: 2024-05-29)

主引用文献

Kim, K.H.,Willingmann, P.,Gong, Z.X.,Kremer, M.J.,Chapman, M.S.,Minor, I.,Oliveira, M.A.,Rossmann, M.G.,Andries, K.,Diana, G.D.,Dutko, F.J.,McKinlay, M.A.,Pevear, D.C.
A comparison of the anti-rhinoviral drug binding pocket in HRV14 and HRV1A.
J.Mol.Biol., 230:206-227, 1993

PubMed Abstract: The three-dimensional structures of two human rhinovirus serotypes (HRV14 and HRV1A) are compared when complexed with various antiviral agents. Although these agents all bind into the same hydrophobic pocket, the exact viral-drug interactions differ. In the absence of drugs, the pocket is occupied by a fatty acid in HRV1A, but is empty in HRV14 except for two water molecules. The conformation of each drug is dependent upon the shape of the hydrophobic pocket. In HRV14 the major residues determining the shape of the binding site are Y1128, P1174 and M1224, corresponding to I1125, M1169 and I1220 in HRV1A. When there is no cofactor or a drug in the pocket, the entrance to the pocket is open. However, the entrance is closed when the pocket is occupied by a cofactor or a drug. There are relatively small conformational changes when the agents displace the natural cofactor in HRV1A. In contrast, there are much larger conformational changes on binding a drug in HRV14. These differences cause an inhibition of viral attachment in HRV14 but not in HRV1A. Binding of the drugs results in three additional interprotomer hydrogen bonds in HRV14 and one in HRV1A. These hydrogen bonds and a potential loss of flexibility upon efficient packing of the pocket may contribute to the inhibition of uncoating in both serotypes.

PubMed: 8383771
DOI: 10.1006/jmbi.1993.1137

実験手法

X-RAY DIFFRACTION (3.8 Å)