2HVE

S120G mutant of human nucleoside diphosphate kinase A complexed with ADP

Summary for 2HVE

• Entry DOI: 10.2210/pdb2hve/pdb
• Related: 1JXV 1UCN 2HVD
• Descriptor: Nucleoside diphosphate kinase A, ADENOSINE-5'-DIPHOSPHATE (3 entities in total)
• Functional Keywords: s120g mutant; complex adp, signaling protein, transferase
• Biological source: Homo sapiens (human)
• Cellular location: Cytoplasm : P15531
• Total number of polymer chains: 3
• Total formula weight: 52703.68

Authors

Giraud, M.-F.,Georgescauld, F.,Lascu, I.,Dautant, A. (deposition date: 2006-07-28, release date: 2006-09-19, Last modification date: 2023-08-30)

Primary citation

Giraud, M.-F.,Georgescauld, F.,Lascu, I.,Dautant, A.
Crystal Structures of S120G Mutant and Wild Type of Human Nucleoside Diphosphate Kinase A in Complex with ADP
J.Bioenerg.Biomembr., 38:261-264, 2006

PubMed Abstract: Nm23 was the first metastasis suppressor gene identified. This gene encodes a NDP kinase that also exhibits other properties like histidine protein kinase and interactions with proteins and DNA. The S120G mutant of NDPK-A has been identified in aggressive neuroblastomas and has been found to reduce the metastasis suppressor effect of Nm23. In order to understand the differences between the wild type and the S120G mutant, we have determined the structure of both mutant and wild type NDPK-A in complex with ADP. Our results reveal that there are no significant changes between the two enzyme versions even in the surroundings of the catalytic histidine that is required for NDP kinase activity. This suggests that the S120G mutation may affect an other protein property than NDP kinase activity.

PubMed: 16944299
DOI: 10.1007/s10863-006-9043-0

Experimental method

X-RAY DIFFRACTION (2.402 Å)