2HUA

Solution Structure of CSFV IRES Domain IIa

2HUA の概要

• エントリーDOI: 10.2210/pdb2hua/pdb
• 分子名称: CSFV IRES Domain IIa (1 entity in total)
• 機能のキーワード: rna hairpin, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12918.78

構造登録者

Locker, N.,Easton, L.E.,Lukavsky, P.J. (登録日: 2006-07-26, 公開日: 2007-04-17, 最終更新日: 2024-05-29)

主引用文献

Locker, N.,Easton, L.E.,Lukavsky, P.J.
HCV and CSFV IRES domain II mediate eIF2 release during 80S ribosome assembly.
Embo J., 26:795-805, 2007

PubMed Abstract: Internal ribosome entry site (IRES) RNAs from the hepatitis C virus (HCV) and classical swine fever virus (CSFV) coordinate cap-independent assembly of eukaryotic 48S initiation complexes, consisting of the 40S ribosomal subunit, eukaryotic initiation factor (eIF) 3 and the eIF2/GTP/Met-tRNA(i)(Met) ternary complex. Here, we report that these IRESes also play a functional role during 80S ribosome assembly downstream of 48S complex formation, in promoting eIF5-induced GTP hydrolysis and eIF2/GDP release from the initiation complex. We show that this function is encoded in their independently folded IRES domain II and that it depends both on its characteristic bent conformation and two conserved RNA motifs, an apical hairpin loop and a loop E. Our data suggest a general mode of subunit joining in HCV and HCV-like IRESes.

PubMed: 17255934
DOI: 10.1038/sj.emboj.7601549

実験手法

SOLUTION NMR