2HT6

Crystal structure of Human Gem G-domain bound to GDP

2HT6 の概要

• エントリーDOI: 10.2210/pdb2ht6/pdb
• 分子名称: GTP-binding protein GEM, MAGNESIUM ION, GUANOSINE-5'-DIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: small g-protein, signaling protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cell membrane; Peripheral membrane protein; Cytoplasmic side: P55040
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 40159.39

構造登録者

Opatowsky, Y.,Hirsch, J.A. (登録日: 2006-07-25, 公開日: 2006-10-31, 最終更新日: 2024-02-14)

主引用文献

Opatowsky, Y.,Sasson, Y.,Shaked, I.,Ward, Y.,Chomsky-Hecht, O.,Litvak, Y.,Selinger, Z.,Kelly, K.,Hirsch, J.A.
Structure-function studies of the G-domain from human gem, a novel small G-protein.
Febs Lett., 580:5959-5964, 2006

PubMed Abstract: Gem, a member of the Rad,Gem/Kir subfamily of small G-proteins, has unique sequence features. We report here the crystallographic structure determination of the Gem G-domain in complex with nucleotide to 2.4 A resolution. Although the basic Ras protein fold is maintained, the Gem switch regions emphatically differ from the Ras paradigm. Our ensuing biochemical characterization indicates that Gem G-domain markedly prefers GDP over GTP. Two known functions of Gem are distinctly affected by spatially separated clusters of mutations.

PubMed: 17052716
DOI: 10.1016/j.febslet.2006.09.067

実験手法

X-RAY DIFFRACTION (2.4 Å)