2HSA

Crystal structure of 12-oxophytodienoate reductase 3 (OPR3) from tomato

2HSA の概要

• エントリーDOI: 10.2210/pdb2hsa/pdb
• 関連するPDBエントリー: 2HS6 2HS8
• 分子名称: 12-oxophytodienoate reductase 3, SULFATE ION, FLAVIN MONONUCLEOTIDE, ... (5 entities in total)
• 機能のキーワード: alpha beta 8 barrel, flavoprotein, jasmonate biosynthesis, oxidoreductase
• 由来する生物種: Solanum lycopersicum
• 細胞内の位置: Peroxisome: Q9FEW9
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 89942.67

構造登録者

Breithaupt, C.,Clausen, T.,Huber, R. (登録日: 2006-07-21, 公開日: 2006-09-12, 最終更新日: 2024-02-14)

主引用文献

Breithaupt, C.,Kurzbauer, R.,Lilie, H.,Schaller, A.,Strassner, J.,Huber, R.,Macheroux, P.,Clausen, T.
Crystal structure of 12-oxophytodienoate reductase 3 from tomato: Self-inhibition by dimerization.
Proc.Natl.Acad.Sci.Usa, 103:14337-14342, 2006

PubMed Abstract: 12-Oxophytodienoate reductase (OPR) 3, a homologue of old yellow enzyme (OYE), catalyzes the reduction of 9S,13S-12-oxophytodienoate to the corresponding cyclopentanone, which is subsequently converted to the plant hormone jasmonic acid (JA). JA and JA derivatives, as well as 12-oxophytodienoate and related cyclopentenones, are known to regulate gene expression in plant development and defense. Together with other oxygenated fatty acid derivatives, they form the oxylipin signature in plants, which resembles the pool of prostaglandins in animals. Here, we report the crystal structure of OPR3 from tomato and of two OPR3 mutants. Although the catalytic residues of OPR3 and related OYEs are highly conserved, several characteristic differences can be discerned in the substrate-binding regions, explaining the remarkable substrate stereoselectivity of OPR isozymes. Interestingly, OPR3 crystallized as an extraordinary self-inhibited dimer. Mutagenesis studies and biochemical analysis confirmed a weak dimerization of OPR3 in vitro, which correlated with a loss of enzymatic activity. Based on structural data of OPR3, a putative mechanism for a strong and reversible dimerization of OPR3 in vivo that involves phosphorylation of OPR3 is suggested. This mechanism could contribute to the shaping of the oxylipin signature, which is critical for fine-tuning gene expression in plants.

PubMed: 16983071
DOI: 10.1073/pnas.0606603103

実験手法

X-RAY DIFFRACTION (1.5 Å)