2HQN

Structure of a Atypical Orphan Response Regulator Protein Revealed a New Phosphorylation-Independent Regulatory Mechanism

2HQN の概要

• エントリーDOI: 10.2210/pdb2hqn/pdb
• 関連するPDBエントリー: 2HQO 2HQR
• 分子名称: Putative TRANSCRIPTIONAL REGULATOR (1 entity in total)
• 機能のキーワード: phosporylation-independent response regulator, signaling protein
• 由来する生物種: Helicobacter pylori
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12481.29

構造登録者

Hong, E.,Lee, W. (登録日: 2006-07-19, 公開日: 2007-05-08, 最終更新日: 2024-05-01)

主引用文献

Hong, E.,Lee, H.M.,Ko, H.,Kim, D.-U.,Jeon, B.-Y.,Jung, J.,Shin, J.,Lee, S.-A.,Kim, Y.,Jeon, Y.H.,Cheong, C.,Cho, H.-S.,Lee, W.
Structure of an Atypical Orphan Response Regulator Protein Supports a New Phosphorylation-independent Regulatory Mechanism
J.Biol.Chem., 282:20667-20675, 2007

PubMed Abstract: Two-component signal transduction systems, commonly found in prokaryotes, typically regulate cellular functions in response to environmental conditions through a phosphorylation-dependent process. A new type of response regulator, hp1043 (HP-RR) from Helicobacter pylori, has been recently identified. HP-RR is essential for cell growth and does not require the well known phosphorelay scheme. Unphosphorylated HP-RR binds specifically to its own promoter (P(1043)) and autoregulates the promoter of the tlpB gene (P(tlpB)). We have determined the structure of HP-RR by NMR and x-ray crystallography, revealing a symmetrical dimer with two functional domains. The molecular topology resembles that of the OmpR/PhoB subfamily, however, the symmetrical dimer is stable even in the unphosphorylated state. The dimer interface, formed by three secondary structure elements (alpha4-beta5-alpha5), resembles that of the active, phosphorylated forms of ArcA and PhoB. Several conserved residues of the HP-RR dimeric interface deviate from the OmpR/PhoB subfamily, although there are similar salt bridges and hydrophobic patches within the interface. Our findings reveal how a new type of response regulator protein could function as a cell growth-associated regulator in the absence of post-translational modification.

PubMed: 17491010
DOI: 10.1074/jbc.M609104200

実験手法

SOLUTION NMR