2HQA

Crystal structure of the catalytic alpha subunit of E. Coli replicative DNA polymerase III

2HQA の概要

• エントリーDOI: 10.2210/pdb2hqa/pdb
• 関連するPDBエントリー: 2HNH
• 分子名称: DNA polymerase III alpha subunit, PHOSPHATE ION (3 entities in total)
• 機能のキーワード: dna polymerase iii, dna replication, nucleotidyltransferase, pol beta, php, transferase
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cytoplasm: P10443
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 104189.99

構造登録者

Lamers, M.H.,Georgescu, R.E.,Lee, S.G.,O'Donnell, M.,Kuriyan, J. (登録日: 2006-07-18, 公開日: 2006-09-19, 最終更新日: 2024-11-13)

主引用文献

Lamers, M.H.,Georgescu, R.E.,Lee, S.G.,O'Donnell, M.,Kuriyan, J.
Crystal Structure of the Catalytic alpha Subunit of E. coli Replicative DNA Polymerase III.
Cell(Cambridge,Mass.), 126:881-892, 2006

PubMed Abstract: Bacterial replicative DNA polymerases such as Polymerase III (Pol III) share no sequence similarity with other polymerases. The crystal structure, determined at 2.3 A resolution, of a large fragment of Pol III (residues 1-917), reveals a unique chain fold with localized similarity in the catalytic domain to DNA polymerase beta and related nucleotidyltransferases. The structure of Pol III is strikingly different from those of members of the canonical DNA polymerase families, which include eukaryotic replicative polymerases, suggesting that the DNA replication machinery in bacteria arose independently. A structural element near the active site in Pol III that is not present in nucleotidyltransferases but which resembles an element at the active sites of some canonical DNA polymerases suggests that, at a more distant level, all DNA polymerases may share a common ancestor. The structure also suggests a model for interaction of Pol III with the sliding clamp and DNA.

PubMed: 16959568
DOI: 10.1016/j.cell.2006.07.028

実験手法

X-RAY DIFFRACTION (2.6 Å)