2HP1

Inter-subunit signaling in GSAM

2HP1 の概要

• エントリーDOI: 10.2210/pdb2hp1/pdb
• 関連するPDBエントリー: 2HOY 2HOZ 2HP2
• 分子名称: Glutamate-1-semialdehyde 2,1-aminomutase (GSAM) plp-form, (4S)-4-AMINO-5-[({3-HYDROXY-2-METHYL-5-[(PHOSPHONOOXY)METHYL]PYRIDIN-4-YL}METHYL)AMINO]PENTANOIC ACID, PYRIDOXAL-5'-PHOSPHATE, ... (5 entities in total)
• 機能のキーワード: inter-subunit signaling, isomerase
• 由来する生物種: Synechococcus elongatus
• 細胞内の位置: Cytoplasm (Potential): P24630
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 92861.65

構造登録者

Stetefeld, J. (登録日: 2006-07-17, 公開日: 2006-08-22, 最終更新日: 2025-03-26)

主引用文献

Stetefeld, J.,Jenny, M.,Burkhard, P.
Intersubunit signaling in glutamate-1-semialdehyde-aminomutase.
Proc.Natl.Acad.Sci.Usa, 103:13688-13693, 2006

PubMed Abstract: Enzymes are highly dynamic and tightly controlled systems. However, allosteric communication linked to catalytic turnover is poorly understood. We have performed an integrated approach to trap several catalytic intermediates in the alpha2-dimeric key enzyme of chlorophyll biosynthesis, glutamate-1-semialdehyde aminomutase. Our data reveal an active-site "gating loop," which undergoes a dramatic conformational change during catalysis, that is simultaneously open in one subunit and closed in the other. This loop movement requires a beta-sheet-to-alpha-helix transition to assume the closed conformation, thus facilitating transport of substrate toward, and concomitantly forming, an integral part of the active site. The accompanying intersubunit cross-talk, which controls negative cooperativity between the allosteric pair, was explored at the atomic level. The central elements of the communication triad are the cofactor bound to different catalytic intermediates, the interface helix, and the gating loop. Together, they form a molecular switch in which the cofactor acts as a central signal transmitter linking the subunit interface with the gating loop.

PubMed: 16954186
DOI: 10.1073/pnas.0600306103

実験手法

X-RAY DIFFRACTION (2.08 Å)