2HOA

STRUCTURE DETERMINATION OF THE ANTP(C39->S) HOMEODOMAIN FROM NUCLEAR MAGNETIC RESONANCE DATA IN SOLUTION USING A NOVEL STRATEGY FOR THE STRUCTURE CALCULATION WITH THE PROGRAMS DIANA, CALIBA, HABAS AND GLOMSA

2HOA の概要

• エントリーDOI: 10.2210/pdb2hoa/pdb
• 分子名称: ANTENNAPEDIA PROTEIN (1 entity in total)
• 機能のキーワード: dna-binding protein, dna binding protein
• 由来する生物種: Drosophila melanogaster (fruit fly)
• 細胞内の位置: Nucleus (Probable): P02833
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8661.09

構造登録者

Guntert, P.,Qian, Y.-Q.,Otting, G.,Muller, M.,Gehring, W.J.,Wuthrich, K. (登録日: 1992-04-04, 公開日: 1993-10-31, 最終更新日: 2024-05-29)

主引用文献

Guntert, P.,Qian, Y.Q.,Otting, G.,Muller, M.,Gehring, W.,Wuthrich, K.
Structure determination of the Antp (C39----S) homeodomain from nuclear magnetic resonance data in solution using a novel strategy for the structure calculation with the programs DIANA, CALIBA, HABAS and GLOMSA.
J.Mol.Biol., 217:531-540, 1991

PubMed Abstract: The structure of a mutant Antennapedia homeodomain, Antp(C39----S), from Drosophila melanogaster was determined using a set of new programs introduced in the accompanying paper. An input dataset of 957 distance constraints and 171 dihedral angle constraints was collected using two-dimensional n.m.r. experiments with the 15N-labeled protein. The resulting high quality structure for Antp(C39----S), with an average root-mean-square deviation of 0.53 A between the backbone atoms of residues 7 to 59 in 20 energy-refined distance geometry structures and the mean structure, is nearly identical to the previously reported structure of the wild-type Antp homeodomain. The only significant difference is in the connection between helices III and IV, which was found to be less kinked than was indicated by the structure determination for Antp. The main emphasis of the presentation in this paper is on a detailed account of the practical use of a novel strategy for the computation of nuclear magnetic resonance structures of proteins with the combined use of the programs DIANA, CALIBA, HABAS and GLOMSA.

PubMed: 1671604
DOI: 10.1016/0022-2836(91)90755-U

実験手法

SOLUTION NMR