2HO7

Pre-cleavage state of the Thermoanaerobacter tengcongensis glmS ribozyme bound to glucose-6-phosphate

2HO7 の概要

• エントリーDOI: 10.2210/pdb2ho7/pdb
• 関連するPDBエントリー: 2HO6
• 分子名称: glmS ribozyme substrate RNA, glmS ribozyme RNA, 6-O-phosphono-alpha-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: rna; ribozyme; pseudoknot; helix, rna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 49564.59

構造登録者

Klein, D.J.,Ferre-D'Amare, A.R. (登録日: 2006-07-13, 公開日: 2006-09-26, 最終更新日: 2024-02-14)

主引用文献

Klein, D.J.,Ferre-D'Amare, A.R.
Structural basis of glmS ribozyme activation by glucosamine-6-phosphate
Science, 313:1752-1756, 2006

PubMed Abstract: The glmS ribozyme is the only natural catalytic RNA known to require a small-molecule activator for catalysis. This catalytic RNA functions as a riboswitch, with activator-dependent RNA cleavage regulating glmS messenger RNA expression. We report crystal structures of the glmS ribozyme in precleavage states that are unliganded or bound to the competitive inhibitor glucose-6-phosphate and in the postcleavage state. All structures superimpose closely, revealing a remarkably rigid RNA that contains a preformed active and coenzyme-binding site. Unlike other riboswitches, the glmS ribozyme binds its activator in an open, solvent-accessible pocket. Our structures suggest that the amine group of the glmS ribozyme-bound coenzyme performs general acid-base and electrostatic catalysis.

PubMed: 16990543
DOI: 10.1126/science.1129666

実験手法

X-RAY DIFFRACTION (2.9 Å)