2HMT

Diamond-shaped octameric ring structure of an RCK domain with NADH bound

2HMT の概要

• エントリーDOI: 10.2210/pdb2hmt/pdb
• 関連するPDBエントリー: 1LNQ 1LSS 1LSU 2HMS 2HMU 2HMV 2HMW
• 分子名称: YuaA protein, 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE (3 entities in total)
• 機能のキーワード: rck, ktn, ktr, ktra, ktrab, membrane protein, ion transporter, symporter, transport protein
• 由来する生物種: Bacillus subtilis
• 細胞内の位置: Cell membrane ; Peripheral membrane protein ; Cytoplasmic side : O32080
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 33483.78

構造登録者

Albright, R.A.,Morais-Cabral, J.H. (登録日: 2006-07-11, 公開日: 2006-09-26, 最終更新日: 2023-08-30)

主引用文献

Albright, R.A.,Ibar, J.L.,Kim, C.U.,Gruner, S.M.,Morais-Cabral, J.H.
The RCK Domain of the KtrAB K(+) Transporter: Multiple Conformations of an Octameric Ring.
Cell(Cambridge,Mass.), 126:1147-1159, 2006

PubMed Abstract: The KtrAB ion transporter is a complex of the KtrB membrane protein and KtrA, an RCK domain. RCK domains regulate eukaryotic and prokaryotic membrane proteins involved in K(+) transport. Conflicting functional models have proposed two different oligomeric arrangements for RCK domains, tetramer versus octamer. Our results for the KtrAB RCK domain clearly show an octamer in solution and in the crystal. We determined the structure of this protein in three different octameric ring conformations that resemble the RCK-domain octamer observed in the MthK potassium channel but show striking differences in size and symmetry. We present experimental evidence for the association between one RCK octameric ring and two KtrB membrane proteins. These results provide insights into the quaternary organization of the KtrAB transporter and its mechanism of activation and show that the RCK-domain octameric ring model is generally applicable to other ion-transport systems.

PubMed: 16990138
DOI: 10.1016/j.cell.2006.08.028

実験手法

X-RAY DIFFRACTION (2.2 Å)