2HLD

Crystal structure of yeast mitochondrial F1-ATPase

2HLD の概要

• エントリーDOI: 10.2210/pdb2hld/pdb
• 分子名称: ATP synthase alpha chain, mitochondrial, ATP synthase beta chain, mitochondrial, ATP synthase gamma chain, mitochondrial, ... (9 entities in total)
• 機能のキーワード: atp phosphorylase (h+ transporting), atp synthase, f1fo, f1-atpase, hydrolase
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• 細胞内の位置: Mitochondrion inner membrane: P07251; Mitochondrion: P00830 P38077 Q12165 P21306
• タンパク質・核酸の鎖数: 27
• 化学式量合計: 1119271.55

構造登録者

Kabaleeswaran, V.,Puri, N.,Walker, J.E.,Leslie, A.G.,Mueller, D.M. (登録日: 2006-07-06, 公開日: 2006-11-28, 最終更新日: 2023-08-30)

主引用文献

Kabaleeswaran, V.,Puri, N.,Walker, J.E.,Leslie, A.G.,Mueller, D.M.
Novel features of the rotary catalytic mechanism revealed in the structure of yeast F(1) ATPase.
Embo J., 25:5433-5442, 2006

PubMed Abstract: The crystal structure of yeast mitochondrial F(1) ATPase contains three independent copies of the complex, two of which have similar conformations while the third differs in the position of the central stalk relative to the alpha(3)beta(3) sub-assembly. All three copies display very similar asymmetric features to those observed for the bovine enzyme, but the yeast F(1) ATPase structures provide novel information. In particular, the active site that binds ADP in bovine F(1) ATPase has an ATP analog bound and therefore this structure does not represent the ADP-inhibited form. In addition, one of the complexes binds phosphate in the nucleotide-free catalytic site, and comparison with other structures provides a picture of the movement of the phosphate group during initial binding and subsequent catalysis. The shifts in position of the central stalk between two of the three copies of yeast F(1) ATPase and when these structures are compared to those of the bovine enzyme give new insight into the conformational changes that take place during rotational catalysis.

PubMed: 17082766
DOI: 10.1038/sj.emboj.7601410

実験手法

X-RAY DIFFRACTION (2.8 Å)