2HJK

Crystal Structure of HLA-B5703 and HIV-1 peptide

2HJK の概要

• エントリーDOI: 10.2210/pdb2hjk/pdb
• 関連するPDBエントリー: 2HJL
• 分子名称: HLA class I histocompatibility antigen B-57, Beta-2-microglobulin, Gag protein, ... (4 entities in total)
• 機能のキーワード: b57, hla, hiv, immune system
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Secreted: P61769; Matrix protein p17: Virion (By similarity): Q1M075
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 44549.68

構造登録者

Gillespie, G.M.A.,Stewart-Jones, G.,Rengasamy, J.,Beattie, T.,Bwayo, J.J.,Plummer, F.A. (登録日: 2006-06-30, 公開日: 2006-10-03, 最終更新日: 2024-10-30)

主引用文献

Gillespie, G.M.A.,Stewart-Jones, G.,Rengasamy, J.,Beattie, T.,Bwayo, J.J.,Plummer, F.A.,Kaul, R.,McMichael, A.J.,Easterbrook, P.,Dong, T.,Jones, E.Y.,Rowland-Jones, S.L.
Strong TCR Conservation and Altered T Cell Cross-Reactivity Characterize a B*57-Restricted Immune Response in HIV-1 Infection.
J.Immunol., 177:3893-3902, 2006

PubMed Abstract: HLA-B*57 is associated with slower disease progression to AIDS, and CD8+ T cell responses to B*57-restricted epitopes are thought to contribute to this protective effect. In this study, we evaluate the B*57-restricted p24 KAFSPEVIPMF (KF11) immune response which is immunodominant during chronic infection. Previously, we observed that the KF11 clade variants KGFNPEVIPMF [A2G,S4N] and KAFNPEIIMPF [S4N,V7I], sharing a position 4 mutation, are differentially recognized by KF11-specific T cells. By combining structural and cellular studies, we now demonstrate that the KF11 and [A2G,S4N] epitopes induce distinct functional responses in [A2G,S4N] and KF11-specific T cells, respectively, despite minimal structural differences between the individual B*57-peptide complexes. Recently, we also elucidated the highly distinct structure of KF11 in complex with B*5703, and have now characterized the CD8+ T cell repertoire recognizing this epitope. We now report striking features of TCR conservation both in terms of TCR Valpha and Vbeta chain usage, and throughout the hypervariable region. Collectively, our findings highlight unusual features of the B*5701/B*5703-KF11-specific immune responses which could influence disease progression and that might be important to consider when designing future vaccine regimens.

PubMed: 16951352

実験手法

X-RAY DIFFRACTION (1.85 Å)