2HIT

Reaction centre from Rhodobacter sphaeroides strain R-26.1 complexed with dibrominated phosphatidylethanolamine

2HIT の概要

• エントリーDOI: 10.2210/pdb2hit/pdb
• 関連するPDBエントリー: 2HG3 2HG9 2HH1 2HHK 2HJ6
• 分子名称: Reaction center protein L chain, PHOSPHATE ION, CARDIOLIPIN, ... (16 entities in total)
• 機能のキーワード: photosynthesis, photosynthetic reaction center, lipid binding sites, brominated lipids, membrane protein, photosynthesis-membrane protein complex, photosynthesis/membrane protein
• 由来する生物種: Rhodobacter sphaeroides; 詳細
• 細胞内の位置: Cellular chromatophore membrane; Multi-pass membrane protein: P0C0Y8 P0C0Y9; Cellular chromatophore membrane; Single-pass membrane protein: P0C0Y7
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 106071.06

構造登録者

Roszak, A.W.,Gardiner, A.T.,Isaacs, N.W.,Cogdell, R.J. (登録日: 2006-06-29, 公開日: 2007-03-27, 最終更新日: 2024-04-03)

主引用文献

Roszak, A.W.,Gardiner, A.T.,Isaacs, N.W.,Cogdell, R.J.
Brominated Lipids Identify Lipid Binding Sites on the Surface of the Reaction Center from Rhodobacter sphaeroides.
Biochemistry, 46:2909-2916, 2007

PubMed Abstract: This study describes the use of brominated phospholipids to distinguish between lipid and detergent binding sites on the surface of a typical alpha-helical membrane protein. Reaction centers isolated from Rhodobacter sphaeroides were cocrystallized with added brominated phospholipids. X-ray structural analysis of these crystals has revealed the presence of two lipid binding sites from the characteristic strong X-ray scattering from the bromine atoms. These results demonstrate the usefulness of this approach to mapping lipid binding sites at the surface of membrane proteins.

PubMed: 17315985
DOI: 10.1021/bi062154i

実験手法

X-RAY DIFFRACTION (2.75 Å)