2HIO

HISTONE OCTAMER (CHICKEN), CHROMOSOMAL PROTEIN

2HIO の概要

• エントリーDOI: 10.2210/pdb2hio/pdb
• 関連するPDBエントリー: 1HIO
• 分子名称: PROTEIN (HISTONE H2A), PROTEIN (HISTONE H2B), PROTEIN (HISTONE H3), ... (4 entities in total)
• 機能のキーワード: histone, chromosomal protein, structural protein, dna binding protein
• 由来する生物種: Gallus gallus (chicken); 詳細
• 細胞内の位置: Nucleus: P02263 P84229 P62801
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 54547.81

構造登録者

Arents, G.,Moudrianakis, E.N. (登録日: 1999-06-15, 公開日: 2000-01-12, 最終更新日: 2023-12-27)

主引用文献

Arents, G.,Burlingame, R.W.,Wang, B.C.,Love, W.E.,Moudrianakis, E.N.
The nucleosomal core histone octamer at 3.1 A resolution: a tripartite protein assembly and a left-handed superhelix.
Proc.Natl.Acad.Sci.USA, 88:10148-10152, 1991

PubMed Abstract: The structure of the octameric histone core of the nucleosome has been determined by x-ray crystallography to a resolution of 3.1 A. The histone octamer is a tripartite assembly in which a centrally located (H3-H4)2 tetramer is flanked by two H2A-H2B dimers. It has a complex outer surface; depending on the perspective, the structure appears as a wedge or as a flat disk. The disk represents the planar projection of a left-handed proteinaceous superhelix with approximately 28 A pitch. The diameter of the particle is 65 A and the length is 60 A at its maximum and approximately 10 A at its minimum extension; these dimensions are in agreement with those reported earlier by Klug et al. [Klug, A., Rhodes, D., Smith, J., Finch, J. T. & Thomas, J. O. (1980) Nature (London) 287, 509-516]. The folded histone chains are elongated rather than globular and are assembled in a characteristic "handshake" motif. The individual polypeptides share a common central structural element of the helix-loop-helix type, which we name the histone fold.

PubMed: 1946434
DOI: 10.1073/pnas.88.22.10148

実験手法

X-RAY DIFFRACTION (3.1 Å)