2HFR

solution structure of antimicrobial peptide Fowlicidin 3

2HFR の概要

• エントリーDOI: 10.2210/pdb2hfr/pdb
• 関連するPDBエントリー: 2amn
• 分子名称: Fowlicidin-3 (1 entity in total)
• 機能のキーワード: alpha helix, antimicrobial protein
• 細胞内の位置: Secreted (By similarity): Q2IAL6
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3101.78

構造登録者

Bommineni, Y.R.,Dai, H.,Gong, Y.,Prakash, O.,Zhang, G. (登録日: 2006-06-26, 公開日: 2007-04-17, 最終更新日: 2024-05-29)

主引用文献

Bommineni, Y.R.,Dai, H.,Gong, Y.X.,Soulages, J.L.,Fernando, S.C.,Desilva, U.,Prakash, O.,Zhang, G.
Fowlicidin-3 is an alpha-helical cationic host defense peptide with potent antibacterial and lipopolysaccharide-neutralizing activities.
Febs J., 274:418-428, 2007

PubMed Abstract: Cathelicidins are an important family of cationic host defense peptides in vertebrates with both antimicrobial and immunomodulatory activities. Fowlicidin-1 and fowlicidin-2 are two newly identified chicken cathelicidins with potent antibacterial activities. Here we report structural and functional characterization of the putatively mature form of the third chicken cathelicidin, fowlicidin-3, for exploration of its therapeutic potential. NMR spectroscopy revealed that fowlicidin-3 comprises 27 amino-acid residues and adopts a predominantly alpha-helical structure extending from residue 9 to 25 with a slight kink induced by a glycine at position 17. It is highly potent against a broad range of Gram-negative and Gram-positive bacteria in vitro, including antibiotic-resistant strains, with minimum inhibitory concentrations in the range 1-2 microM. It kills bacteria quickly, permeabilizing cytoplasmic membranes immediately on coming into contact with them. Unlike many other host defense peptides with antimicrobial activities that are diminished by serum or salt, fowlicidin-3 retains bacteria-killing activities in the presence of 50% serum or physiological concentrations of salt. Furthermore, it is capable of suppressing lipopolysaccharide-induced expression of proinflammatory genes in mouse macrophage RAW264.7 cells, with nearly complete blockage at 10 microM. Fowlicidin-3 appears to be an excellent candidate for future development as a novel antimicrobial and antisepsis agent, particularly against antibiotic-resistant pathogens.

PubMed: 17229147
DOI: 10.1111/j.1742-4658.2006.05589.x

実験手法

SOLUTION NMR