2HEK

Crystal structure of O67745, a hypothetical protein from Aquifex aeolicus at 2.0 A resolution.

Summary for 2HEK

• Entry DOI: 10.2210/pdb2hek/pdb
• Descriptor: Hypothetical protein, PHOSPHATE ION, ZINC ION, ... (8 entities in total)
• Functional Keywords: predominantly alpha helical protein with gdp binding site and active site being far from each other, structural genomics, psi, protein structure initiative, berkeley structural genomics center, bsgc, unknown function
• Biological source: Aquifex aeolicus
• Total number of polymer chains: 2
• Total formula weight: 90764.41

Authors

Oganesyan, V.,Jancarik, J.,Adams, P.D.,Kim, R.,Kim, S.H.,Berkeley Structural Genomics Center (BSGC) (deposition date: 2006-06-21, release date: 2006-07-04, Last modification date: 2024-02-14)

Primary citation

Oganesyan, V.,Adams, P.D.,Jancarik, J.,Kim, R.,Kim, S.H.
Structure of O67745_AQUAE, a hypothetical protein from Aquifex aeolicus.
Acta Crystallogr.,Sect.F, 63:369-374, 2007

PubMed Abstract: Using single-wavelength anomalous dispersion data obtained from a gold-derivatized crystal, the X-ray crystal structure of the protein 067745_AQUAE from the prokaryotic organism Aquifex aeolicus has been determined to a resolution of 2.0 A. Amino-acid residues 1-371 of the 44 kDa protein were identified by Pfam as an HD domain and a member of the metal-dependent phosphohydrolase superfamily (accession No. PF01966). Although three families from this large and diverse group of enzymatic proteins are represented in the PDB, the structure of 067745_AQUAE reveals a unique fold that is unlike the others and that is likely to represent a new subfamily, further organizing the families and characterizing the proteins. Data are presented that provide the first insights into the structural organization of the proteins within this clan and a distal alternative GDP-binding domain outside the metal-binding active site is proposed.

PubMed: 17565173
DOI: 10.1107/S1744309107018945

Experimental method

X-RAY DIFFRACTION (1.997 Å)