2HDF

Crystal structure of the Colicin I receptor Cir from E.coli

2HDF の概要

• エントリーDOI: 10.2210/pdb2hdf/pdb
• 関連するPDBエントリー: 2HDI
• 分子名称: Colicin I receptor, STRONTIUM ION, N-OCTYL-2-HYDROXYETHYL SULFOXIDE, ... (4 entities in total)
• 機能のキーワード: outer membrane, iron transport, tonb box, signal transduction, colicin i receptor, membrane protein, protein transport
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cell outer membrane: P17315
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 72263.51

構造登録者

Buchanan, S.K.,Esser, L.,Lukacik, P. (登録日: 2006-06-20, 公開日: 2007-05-08, 最終更新日: 2024-11-06)

主引用文献

Buchanan, S.K.,Lukacik, P.,Grizot, S.,Ghirlando, R.,Ali, M.M.,Barnard, T.J.,Jakes, K.S.,Kienker, P.K.,Esser, L.
Structure of colicin I receptor bound to the R-domain of colicin Ia: implications for protein import.
Embo J., 26:2594-2604, 2007

PubMed Abstract: Colicin Ia is a 69 kDa protein that kills susceptible Escherichia coli cells by binding to a specific receptor in the outer membrane, colicin I receptor (70 kDa), and subsequently translocating its channel forming domain across the periplasmic space, where it inserts into the inner membrane and forms a voltage-dependent ion channel. We determined crystal structures of colicin I receptor alone and in complex with the receptor binding domain of colicin Ia. The receptor undergoes large and unusual conformational changes upon colicin binding, opening at the cell surface and positioning the receptor binding domain of colicin Ia directly above it. We modelled the interaction with full-length colicin Ia to show that the channel forming domain is initially positioned 150 A above the cell surface. Functional data using full-length colicin Ia show that colicin I receptor is necessary for cell surface binding, and suggest that the receptor participates in translocation of colicin Ia across the outer membrane.

PubMed: 17464289
DOI: 10.1038/sj.emboj.7601693

実験手法

X-RAY DIFFRACTION (2.65 Å)