2HDC

STRUCTURE OF TRANSCRIPTION FACTOR GENESIS/DNA COMPLEX

2HDC の概要

• エントリーDOI: 10.2210/pdb2hdc/pdb
• 分子名称: DNA (5'-D(P*GP*CP*TP*TP*AP*AP*AP*AP*TP*AP*AP*CP*AP*AP*TP*AP*C)-3'), DNA (5'-D(P*GP*TP*AP*TP*TP*GP*TP*TP*AP*TP*TP*TP*TP*AP*AP*GP*C)-3'), PROTEIN (TRANSCRIPTION FACTOR) (3 entities in total)
• 機能のキーワード: dyanamics, genesis, winged helix protein, protein-dna complex, protein/dna
• 由来する生物種: Rattus norvegicus (Norway rat)
• 細胞内の位置: Nucleus (By similarity): Q63245
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 21823.94

構造登録者

Jin, C.,Marsden, I.,Chen, X.,Liao, X. (登録日: 1999-05-05, 公開日: 1999-07-05, 最終更新日: 2023-12-27)

主引用文献

Jin, C.,Marsden, I.,Chen, X.,Liao, X.
Dynamic DNA contacts observed in the NMR structure of winged helix protein-DNA complex.
J.Mol.Biol., 289:683-690, 1999

PubMed Abstract: Genesis is an HNF-3/fkh homologous protein. By using multi-dimensional NMR techniques, we have obtained the solution structure and backbone dynamics of Genesis complexed with a 17 base-pair DNA. Our results indicate that both the local folding and dynamic properties of Genesis are perturbed when it binds to the DNA site. Our data show that a conserved flexible amino acid sequence (wing 1) makes dynamic contacts to DNA in the complex and a short helix is induced by Genesis-DNA interactions. Our data indicate that, unlike the HNF-3gamma/DNA complex, a magnesium ion is not required in forming the stable Genesis-DNA complex.

PubMed: 10369754
DOI: 10.1006/jmbi.1999.2819

実験手法

SOLUTION NMR