2HD7

Solution structure of C-teminal domain of twinfilin-1.

2HD7 の概要

• エントリーDOI: 10.2210/pdb2hd7/pdb
• 分子名称: Twinfilin-1 (1 entity in total)
• 機能のキーワード: adf-h, actin binding protein, contractile protein
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Cytoplasm: Q91YR1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16638.95

構造登録者

Hellman, M.H.,Paavilainen, V.O.,Annila, A.,Lappalainen, P.,Permi, P.I. (登録日: 2006-06-20, 公開日: 2007-02-06, 最終更新日: 2024-05-29)

主引用文献

Paavilainen, V.O.,Hellman, M.,Helfer, E.,Bovellan, M.,Annila, A.,Carlier, M.F.,Permi, P.,Lappalainen, P.
Structural basis and evolutionary origin of actin filament capping by twinfilin
Proc.Natl.Acad.Sci.Usa, 104:3113-3118, 2007

PubMed Abstract: Dynamic reorganization of the actin cytoskeleton is essential for motile and morphological processes in all eukaryotic cells. One highly conserved protein that regulates actin dynamics is twinfilin, which both sequesters actin monomers and caps actin filament barbed ends. Twinfilin is composed of two ADF/cofilin-like domains, Twf-N and Twf-C. Here, we reveal by systematic domain-swapping/inactivation analysis that the two functional ADF-H domains of twinfilin are required for barbed-end capping and that Twf-C plays a critical role in this process. However, these domains are not functionally equivalent. NMR-structure and mutagenesis analyses, together with biochemical and motility assays showed that Twf-C, in addition to its binding to G-actin, interacts with the sides of actin filaments like ADF/cofilins, whereas Twf-N binds only G-actin. Our results indicate that during filament barbed-end capping, Twf-N interacts with the terminal actin subunit, whereas Twf-C binds between two adjacent subunits at the side of the filament. Thus, the domain requirement for actin filament capping by twinfilin is remarkably similar to that of gelsolin family proteins, suggesting the existence of a general barbed-end capping mechanism. Furthermore, we demonstrate that a synthetic protein consisting of duplicated ADF/cofilin domains caps actin filament barbed ends, providing evidence that the barbed-end capping activity of twinfilin arose through a duplication of an ancient ADF/cofilin-like domain.

PubMed: 17360616
DOI: 10.1073/pnas.0608725104

実験手法

SOLUTION NMR