2HBN

Crystallization of the Tl+-form of the Oxytricha nova G-quadruplex

2HBN の概要

• エントリーDOI: 10.2210/pdb2hbn/pdb
• 関連するPDBエントリー: 1JRN 2AKG
• 分子名称: 5'-D(*GP*GP*GP*GP*TP*TP*TP*TP*GP*GP*GP*G)-3', THALLIUM (I) ION (3 entities in total)
• 機能のキーワード: deoxyribonucleic acid, g-quadruplex, thallium, dna
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 17265.67

構造登録者

Gill, M.L.,Strobel, S.A.,Loria, J.P. (登録日: 2006-06-14, 公開日: 2006-09-19, 最終更新日: 2023-08-30)

主引用文献

Gill, M.L.,Strobel, S.A.,Loria, J.P.
Crystallization and characterization of the thallium form of the Oxytricha nova G-quadruplex.
Nucleic Acids Res., 34:4506-4514, 2006

PubMed Abstract: The crystal structure of the Tl+ form of the G-quadruplex formed from the Oxytricha nova telomere sequence, d(G4T4G4), has been solved to 1.55 A. This G-quadruplex contains five Tl+ ions, three of which are interspersed between adjacent G-quartet planes and one in each of the two thymine loops. The structure displays a high degree of similarity to the K+ crystal structure [Haider et al. (2002), J. Mol. Biol., 320, 189-200], including the number and location of the monovalent cation binding sites. The highly isomorphic nature of the two structures, which contain such a large number of monovalent binding sites (relative to nucleic acid content), verifies the ability of Tl+ to mimic K+ in nucleic acids. Information from this report confirms and extends the assignment of 205Tl resonances from a previous report [Gill et al. (2005), J. Am. Chem. Soc., 127, 16 723-16 732] where 205Tl NMR was used to study monovalent cation binding to this G-quadruplex. The assignment of these resonances provides evidence for the occurrence of conformational dynamics in the thymine loop region that is in slow exchange on the 205Tl timescale.

PubMed: 16945956
DOI: 10.1093/nar/gkl616

実験手法

X-RAY DIFFRACTION (1.55 Å)