2H8C

Structure of RusA D70N in complex with DNA

2H8C の概要

• エントリーDOI: 10.2210/pdb2h8c/pdb
• 関連するPDBエントリー: 2H8E
• 分子名称: 5'-D(*CP*CP*GP*GP*TP*AP*CP*CP*GP*GP*T)-3', Crossover junction endodeoxyribonuclease rusA (3 entities in total)
• 機能のキーワード: protein-dna complex, recombination, hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 68868.54

構造登録者

Macmaster, R.A. (登録日: 2006-06-07, 公開日: 2007-04-24, 最終更新日: 2023-08-30)

主引用文献

Macmaster, R.,Sedelnikova, S.,Baker, P.J.,Bolt, E.L.,Lloyd, R.G.,Rafferty, J.B.
RusA Holliday junction resolvase: DNA complex structure--insights into selectivity and specificity.
Nucleic Acids Res., 34:5577-5584, 2006

PubMed Abstract: We have determined the structure of a catalytically inactive D70N variant of the Escherichia coli RusA resolvase bound to a duplex DNA substrate that reveals critical protein-DNA interactions and permits a much clearer understanding of the interaction of the enzyme with a Holliday junction (HJ). The RusA enzyme cleaves HJs, the fourway DNA branchpoints formed by homologous recombination, by introducing symmetrical cuts in the phosphodiester backbone in a Mg2+ dependent reaction. Although, RusA shows a high level of selectivity for DNA junctions, preferring to bind fourway junctions over other substrates in vitro, it has also been shown to have appreciable affinity for duplex DNA. However, RusA does not show DNA cleavage activity with duplex substrates. Our structure suggests the possible basis for structural selectivity as well as sources of the sequence specificity observed for DNA cleavage by RusA.

PubMed: 17028102
DOI: 10.1093/nar/gkl447

実験手法

X-RAY DIFFRACTION (3.1 Å)