2H8A

Structure of Microsomal Glutathione Transferase 1 in Complex with Glutathione

2H8A の概要

• エントリーDOI: 10.2210/pdb2h8a/pdb
• 分子名称: Microsomal glutathione S-transferase 1, GLUTATHIONE (2 entities in total)
• 機能のキーワード: membrane protein, transferase
• 由来する生物種: Rattus norvegicus (Norway rat)
• 細胞内の位置: Microsome: P08011
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17668.61

構造登録者

Hebert, H. (登録日: 2006-06-07, 公開日: 2007-05-22, 最終更新日: 2024-02-14)

主引用文献

Holm, P.J.,Bhakat, P.,Jegerschold, C.,Gyobu, N.,Mitsuoka, K.,Fujiyoshi, Y.,Morgenstern, R.,Hebert, H.
Structural basis for detoxification and oxidative stress protection in membranes.
J.Mol.Biol., 360:934-945, 2006

PubMed Abstract: Synthesis of mediators of fever, pain and inflammation as well as protection against reactive molecules and oxidative stress is a hallmark of the MAPEG superfamily (membrane associated proteins in eicosanoid and glutathione metabolism). The structure of a MAPEG member, rat microsomal glutathione transferase 1, at 3.2 A resolution, solved here in complex with glutathione by electron crystallography, defines the active site location and a cytosolic domain involved in enzyme activation. The glutathione binding site is found to be different from that of the canonical soluble glutathione transferases. The architecture of the homotrimer supports a catalytic mechanism involving subunit interactions and reveals both cytosolic and membraneous substrate entry sites, providing a rationale for the membrane location of the enzyme.

PubMed: 16806268
DOI: 10.1016/j.jmb.2006.05.056

実験手法

ELECTRON CRYSTALLOGRAPHY (3.2 Å)