2H80

NMR structures of SAM domain of Deleted in Liver Cancer 2 (DLC2)

2H80 の概要

• エントリーDOI: 10.2210/pdb2h80/pdb
• NMR情報: BMRB: 7151
• 分子名称: StAR-related lipid transfer protein 13 (1 entity in total)
• 機能のキーワード: helical bundle, lipid binding protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: Q9Y3M8
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9339.57

構造登録者

Li, H.Y.,Fung, K.L.,Jin, D.Y.,Chung, S.S.,Ko, B.C.,Sun, H.Z. (登録日: 2006-06-06, 公開日: 2007-05-15, 最終更新日: 2024-05-29)

主引用文献

Li, H.,Fung, K.L.,Jin, D.Y.,Chung, S.S.,Ching, Y.P.,Ng, I.O.,Sze, K.H.,Ko, B.C.,Sun, H.
Solution structures, dynamics, and lipid-binding of the sterile alpha-motif domain of the deleted in liver cancer 2
Proteins, 67:1154-1166, 2007

PubMed Abstract: The deleted in liver cancer 2 (DLC2) is a tumor suppressor gene, frequently found to be underexpressed in hepatocellular carcinoma. DLC2 is a multidomain protein containing a sterile alpha-motif (SAM) domain, a GTPase-activating protein (GAP) domain, and a lipid-binding StAR-related lipid-transfer (START) domain. The SAM domain of DLC2, DLC2-SAM, exhibits a low level of sequence homology (15-30%) with other SAM domains, and appears to be the prototype of a new subfamily of SAM domains found in DLC2-related proteins. In the present study, we have determined the three-dimensional solution structure of DLC2-SAM using NMR methods together with molecular dynamics simulated annealing. In addition, we performed a backbone dynamics study. The DLC2-SAM packed as a unique four alpha-helical bundle stabilized by interhelix hydrophobic interactions. The arrangement of the four helices is distinct from all other known SAM domains. In contrast to some members of the SAM domain family which form either dimers or oligomers, both biochemical analyses and rotational correlation time (tau(c)) measured by backbone 15N relaxation experiments indicated that DLC2-SAM exists as a monomer in solution. The interaction of DLC2-SAM domain with sodium dodecyl sulfate (SDS) micelles and 1,2-dimyristoyl-sn-glycerol-3-phosphatidylglycerol (DMPG) phospholipids was examined by CD and NMR spectroscopic techniques. The DLC2-SAM exhibits membrane binding properties accompanied by minor loss of the secondary structure of the protein. Deletion studies showed that the self-association of DLC2 in vivo does not require SAM domain, instead, a protein domain consisting of residues 120-672 mediates the self-association of DLC2.

PubMed: 17380510
DOI: 10.1002/prot.21361

実験手法

SOLUTION NMR