2H60

Solution Structure of Human Brg1 Bromodomain

2H60 の概要

• エントリーDOI: 10.2210/pdb2h60/pdb
• 分子名称: Probable global transcription activator SNF2L4 (1 entity in total)
• 機能のキーワード: alfa helix, transcription
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus: P51532
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15188.44

構造登録者

Shen, W.,Xu, C.,Zhang, J.,Wu, J.,Shi, Y. (登録日: 2006-05-30, 公開日: 2007-02-13, 最終更新日: 2024-05-29)

主引用文献

Shen, W.,Xu, C.,Huang, W.,Zhang, J.,Carlson, J.E.,Tu, X.,Wu, J.,Shi, Y.
Solution structure of human Brg1 bromodomain and its specific binding to acetylated histone tails
Biochemistry, 46:2100-2110, 2007

PubMed Abstract: Human brahma-related gene 1 (Brg1) is a core protein in human SWI/SNF chromatin-remodeling complex which regulates gene expression. Brg1 contains a bromodomain that has been shown to anchor the entire complex to promoter nucleosomes by interacting with histones that are acetylated at specific lysine residues. The Brg1 bromodomain belongs to an important subclass of the bromodomain family for which no structural information is known. Here we report the solution structure of the Brg1 bromodomain determined by NMR. The Brg1 bromodomain conserves the left-handed, four-helix bundle topology found in other bromodomain structures. However, the alphaZ helix of Brg1 bromodomain is about 4 residues shorter relative to previously published bromodomain structures. Using NMR perturbation studies, we demonstrate the Brg1 bromodomain binds acetyllysine in the context of histone tails, with no comparable affinity for unacetylated peptides. The estimated dissociation constants (KD) for acetylated histone peptides H4-AcK8 and H4-AcK12 are 4.0 and 3.6 mM, respectively. In this study the dominant substrate was H3-AcK14 (KD approximately 1.2 mM). Mutagenesis analysis reveals several residues important for the binding specificity. Using molecular dynamics simulations, we present a model of the Brg1 bromodomain in complex with H3-AcK14 and discuss the potential interactions which provide the selectivity of the Brg1 bromodomain for histone H3-AcK14.

PubMed: 17274598
DOI: 10.1021/bi0611208

実験手法

SOLUTION NMR