2H3K

Solution Structure of the first NEAT domain of IsdH

Summary for 2H3K

• Entry DOI: 10.2210/pdb2h3k/pdb
• NMR Information: BMRB: 6759
• Descriptor: Haptoglobin-binding surface anchored protein (1 entity in total)
• Functional Keywords: neat domain, isdh, hara, protein binding
• Biological source: Staphylococcus aureus subsp. aureus
• Total number of polymer chains: 1
• Total formula weight: 16733.45

Authors

Pilpa, R.M.,Fadeev, E.A.,Villareal, V.A.,Wong, M.A.,Phillips, M.,Clubb, R.T. (deposition date: 2006-05-22, release date: 2006-08-22, Last modification date: 2024-05-08)

Primary citation

Pilpa, R.M.,Fadeev, E.A.,Villareal, V.A.,Wong, M.L.,Phillips, M.,Clubb, R.T.
Solution structure of the NEAT (NEAr Transporter) domain from IsdH/HarA: the human hemoglobin receptor in Staphylococcus aureus.
J.Mol.Biol., 360:435-447, 2006

PubMed Abstract: During infections the pathogen Staphylococcus aureus procures the essential nutrient iron from its host using iron-regulated surface determinant (Isd) proteins, which scavenge heme bound iron from host hemoproteins. Four Isd proteins are displayed in the cell wall, where they function as receptors for host proteins and heme. Each of the receptors contains one or more copies of a recently discovered domain called NEAT (NEAr Transporter) that has been shown to mediate protein binding. Here we report the three-dimensional solution structure of the NEAT domain from the IsdH/HarA protein, which is the hemoglobin receptor in the Isd system. This is the first structure of a NEAT domain and reveals that they adopt a beta sandwich fold that consists of two five-stranded antiparallel beta sheets. Although unrelated at the primary sequence level, our results indicate that NEAT domains belong to the immunoglobulin superfamily. Binding studies indicate that two IsdH/HarA NEAT domains bind a single molecule of methemoglobin, while the distantly related NEAT domain from the S. aureus IsdC protein binds only heme. A comparison of their primary sequences in light of the new structure is used to predict the hemoglobin and heme binding surfaces on NEAT domains.

PubMed: 16762363
DOI: 10.1016/j.jmb.2006.05.019

Experimental method

SOLUTION NMR