2H2P

Crystal structure of CLC-ec1 in complex with Fab fragment in SeCN-

2H2P の概要

• エントリーDOI: 10.2210/pdb2h2p/pdb
• 関連するPDBエントリー: 1OTS 2H2S
• 分子名称: CLC Cl transporter, FAB fragment, heavy chain, FAB fragment, light chain, ... (4 entities in total)
• 機能のキーワード: clc; transporter; chloride; antiport, ion transport
• 由来する生物種: Escherichia coli; 詳細
• 細胞内の位置: Cell inner membrane ; Multi- pass membrane protein : P37019
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 193721.93

構造登録者

Nguitragool, W.,Miller, C. (登録日: 2006-05-19, 公開日: 2006-05-30, 最終更新日: 2024-10-16)

主引用文献

Nguitragool, W.,Miller, C.
Uncoupling of a CLC Cl(-)/H(+) Exchange Transporter by Polyatomic Anions
J.Mol.Biol., 362:682-690, 2006

PubMed Abstract: CLC-ec1 is a bacterial archetype of CLC transporters, a ubiquitous class of proteins that catalyze transmembrane exchange of Cl- and H+ necessary for pH regulation of numerous physiological processes. Despite a profusion of high-resolution structures, the molecular mechanism of exchange remains unknown. Here, we rigorously demonstrate strict exchange stoichiometry of 2 Cl-/1 H+. In addition to Cl- and Br-, two non-halide ions, NO3- and SCN-, are shown to be transported by CLC-ec1, but with reduced H+ counter-transport. The loss of proton coupling to these anions is accompanied by an absence of bound anions in the central and external Cl- binding sites in the protein's anion selectivity region, as revealed by crystallographic comparison of Br- and SeCN- bound to this region.

PubMed: 16905147
DOI: 10.1016/j.jmb.2006.07.006

実験手法

X-RAY DIFFRACTION (3.1 Å)