2H1Y

Crystal structure of malonyl-CoA:Acyl carrier protein transacylase (MCAT) from Helicobacter pylori

2H1Y の概要

• エントリーDOI: 10.2210/pdb2h1y/pdb
• 分子名称: Malonyl coenzyme A-acyl carrier protein transacylase (2 entities in total)
• 機能のキーワード: fabd, mcat, transferase
• 由来する生物種: Helicobacter pylori
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 71606.52

構造登録者

Zhang, L.,Liu, W.,Shen, X.,Jiang, H. (登録日: 2006-05-17, 公開日: 2007-05-22, 最終更新日: 2023-10-25)

主引用文献

Zhang, L.,Liu, W.,Xiao, J.,Hu, T.,Chen, J.,Chen, K.,Jiang, H.,Shen, X.
Malonyl-CoA: acyl carrier protein transacylase from Helicobacter pylori: Crystal structure and its interaction with acyl carrier protein
Protein Sci., 16:1184-1192, 2007

PubMed Abstract: Malonyl-CoA: acyl carrier protein transacylase (MCAT) is a critical enzyme responsible for the transfer of the malonyl moiety to holo-acyl carrier protein (ACP) forming the malonyl-ACP intermediates in the initiation step of type II fatty acid synthesis (FAS II) in bacteria. MCAT has been considered as an attractive drug target in the discovery of antibacterial agents. In this study, the crystal structure of MCAT from Helicobacter pylori (Hp) at 2.5 angstroms resolution is reported, and the interaction of HpMCAT with HpACP is extensively investigated by using computational docking, GST-pull-down, and surface plasmon resonance (SPR) technology-based assays. The crystal structure results reveal that HpMCAT has a compact folding composed of a large subdomain with a similar core as in alpha/beta hydrolases, and a similar ferredoxin-like small subdomain as in acylphosphatases. The docking result suggests two positively charged areas near the entrance of the active site of HpMCAT as the ACP-binding region. Binding assay research shows that HpMCAT demonstrates a moderately binding ability against HpACP. The solved 3D structure of HpMCAT is expected to supply useful information for the structure-based discovery of novel inhibitors against MCAT, and the quantitative study of HpMCAT interaction with HpACP is hoped to give helpful hints in the understanding of the detailed catalytic mechanisms for HpMCAT.

PubMed: 17525466
DOI: 10.1110/ps.072757307

実験手法

X-RAY DIFFRACTION (2.5 Å)