2GX0

Crystal structural and functional analysis of GFP-like fluorescent protein

2GX0 の概要

• エントリーDOI: 10.2210/pdb2gx0/pdb
• 関連するPDBエントリー: 2GX2
• 分子名称: fluorescent protein Dronpa (2 entities in total)
• 機能のキーワード: fluorescence, b-can, photoswitching, luminescent protein
• 由来する生物種: Echinophyllia sp. SC22 (coral)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 110453.18

構造登録者

Hwang, K.Y.,Nam, K.-H.,Park, S.-Y.,Sugiyama, K. (登録日: 2006-05-08, 公開日: 2007-05-08, 最終更新日: 2026-03-18)

主引用文献

Nam, K.-H.,Kwon, O.Y.,Sugiyama, K.,Lee, W.-H.,Kim, Y.K.,Song, H.K.,Kim, E.E.,Park, S.-Y.,Jeon, H.,Hwang, K.Y.
Structural characterization of the photoswitchable fluorescent protein Dronpa-C62S
Biochem.Biophys.Res.Commun., 354:962-967, 2007

PubMed Abstract: The photoswitching behavior of green fluorescent proteins (GFPs) or GFP-like proteins is increasingly recognized as a new technique for optical marking. Recently, Ando and his colleagues developed a new green fluorescent protein Dronpa, which possesses the unique photochromic property of being photoswitchable in a non-destructive manner. To better understand this mechanism, we determined the crystal structures of a new GFP Dronpa and its mutant C62S, at 1.9 Angstroms and 1.8 Angstroms, respectively. Determination of the structures demonstrates that a unique hydrogen-bonding network and the sulfur atom of the chromophore are critical to the photoswitching property of Dronpa. Reversible photoswitching was lost in cells expressing the Dronpa-C62S upon repetitive irradiation compared to the native protein. Structural and mutational analyses reveal the chemical basis for the functional properties of photoswitchable fluorescent proteins and provide the basis for subsequent coherent engineering of this subfamily of Dronpa homologs.

PubMed: 17276392
DOI: 10.1016/j.bbrc.2007.01.086

実験手法

X-RAY DIFFRACTION (1.9 Å)