2GWL

Crystal structure of the Salmonella SpvB ATR Domain in complex with NADH

2GWL の概要

• エントリーDOI: 10.2210/pdb2gwl/pdb
• 関連するPDBエントリー: 2GWM
• 分子名称: 65 kDa virulence protein, 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE (3 entities in total)
• 機能のキーワード: salmonella, spvb, adp-ribosyltransferase, transferase
• 由来する生物種: Salmonella typhimurium
• 細胞内の位置: Secreted: P17450
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 23281.01

構造登録者

Stebbins, C.E.,Margarit, S.M. (登録日: 2006-05-04, 公開日: 2006-08-29, 最終更新日: 2017-10-18)

主引用文献

Margarit, S.M.,Davidson, W.,Frego, L.,Stebbins, C.E.
A steric antagonism of actin polymerization by a salmonella virulence protein.
Structure, 14:1219-1229, 2006

PubMed Abstract: Salmonella spp. require the ADP-ribosyltransferase activity of the SpvB protein for intracellular growth and systemic virulence. SpvB covalently modifies actin, causing cytoskeletal disruption and apoptosis. We report here the crystal structure of the catalytic domain of SpvB, and we show by mass spectrometric analysis that SpvB modifies actin at Arg177, inhibiting its ATPase activity. We also describe two crystal structures of SpvB-modified, polymerization-deficient actin. These structures reveal that ADP-ribosylation does not lead to dramatic conformational changes in actin, suggesting a model in which this large family of toxins inhibits actin polymerization primarily through steric disruption of intrafilament contacts.

PubMed: 16905096
DOI: 10.1016/j.str.2006.05.022

実験手法

X-RAY DIFFRACTION (1.9 Å)