2GV3

Translocation of a tRNA with an extended anticodon through the ribosome

2GV3 の概要

• エントリーDOI: 10.2210/pdb2gv3/pdb
• 分子名称: 5'-R(*GP*GP*CP*CP*AP*GP*AP*CP*UP*CP*CP*CP*GP*AP*AP*UP*CP*UP*GP*GP*CP*C)-3' (1 entity in total)
• 機能のキーワード: stemloop, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7008.23

構造登録者

Phelps, S.S.,Gaudin, C.,Yoshizawa, S.,Benitez, C.,Fourmy, D.,Joseph, S. (登録日: 2006-05-02, 公開日: 2006-08-08, 最終更新日: 2024-05-29)

主引用文献

Phelps, S.S.,Gaudin, C.,Yoshizawa, S.,Benitez, C.,Fourmy, D.,Joseph, S.
Translocation of a tRNA with an Extended Anticodon Through the Ribosome.
J.Mol.Biol., 360:610-622, 2006

PubMed Abstract: Coordinated translocation of the tRNA-mRNA complex by the ribosome occurs in a precise, stepwise movement corresponding to a distance of three nucleotides along the mRNA. Frameshift suppressor tRNAs generally contain an extra nucleotide in the anticodon loop and they subvert the normal mechanisms used by the ribosome for frame maintenance. The mechanism by which suppressor tRNAs traverse the ribosome during translocation is poorly understood. Here, we demonstrate translocation of a tRNA by four nucleotides from the A site to the P site, and from the P site to the E site. We show that translocation of a punctuated mRNA is possible with an extra, unpaired nucleotide between codons. Interestingly, the NMR structure of the four nucleotide anticodon stem-loop reveals a conformation different from the canonical tRNA structure. Flexibility within the loop may allow conformational adjustment upon A site binding and for interacting with the four nucleotide codon in order to shift the mRNA reading frame.

PubMed: 16787653
DOI: 10.1016/j.jmb.2006.05.016

実験手法

SOLUTION NMR