2GSY

The 2.6A structure of Infectious Bursal Virus Derived T=1 Particles

2GSY の概要

• エントリーDOI: 10.2210/pdb2gsy/pdb
• 分子名称: polyprotein, CALCIUM ION (3 entities in total)
• 機能のキーワード: virus-like capsid (protein-protein complex), icosahedral virus, virus
• 由来する生物種: Infectious bursal disease virus (Gumboro virus)
• 細胞内の位置: Capsid protein VP2: Virion (Potential). Capsid protein VP3: Virion (Potential). Structural peptide 1: Virion (Potential). Structural peptide 2: Virion (Potential). Structural peptide 3: Virion (Potential). Structural peptide 4: Virion (Potential): P61825
• タンパク質・核酸の鎖数: 20
• 化学式量合計: 978685.32

構造登録者

Garriga, D.,Querol-Audi, J.,Abaitua, F.,Saugar, I.,Pous, J.,Verdaguer, N.,Caston, J.R.,Rodriguez, J.F. (登録日: 2006-04-27, 公開日: 2006-07-18, 最終更新日: 2023-08-30)

主引用文献

Garriga, D.,Querol-Audi, J.,Abaitua, F.,Saugar, I.,Pous, J.,Verdaguer, N.,Caston, J.R.,Rodriguez, J.F.
The 2.6-angstrom structure of infectious bursal disease virus-derived t=1 particles reveals new stabilizing elements of the virus capsid.
J.Virol., 80:6895-6905, 2006

PubMed Abstract: Infectious bursal disease virus (IBDV), a member of the Birnaviridae family, is a double-stranded RNA virus that causes a highly contagious disease in young chickens leading to significant economic losses in the poultry industry. The VP2 protein, the only structural component of the IBDV icosahedral capsid, spontaneously assembles into T=1 subviral particles (SVP) when individually expressed as a chimeric gene. We have determined the crystal structure of the T=1 SVP to 2.60 A resolution. Our results show that the 20 trimeric VP2 clusters forming the T=1 shell are further stabilized by calcium ions located at the threefold icosahedral axes. The structure also reveals a new unexpected domain swapping that mediates interactions between adjacent trimers: a short helical segment located close to the end of the long C-terminal arm of VP2 is projected toward the threefold axis of a neighboring VP2 trimer, leading to a complex network of interactions that increases the stability of the T=1 particles. Analysis of crystal packing shows that the exposed capsid residues, His253 and Thr284, determinants of IBDV virulence and the adaptation of the virus to grow in cell culture, are involved in particle-particle interactions.

PubMed: 16809295
DOI: 10.1128/JVI.00368-06

実験手法

X-RAY DIFFRACTION (2.6 Å)